{"title":"从Jalingo大都会内一些选定的食品中心收集的与蟑螂有关的细菌","authors":"Luka Larubi, A. Francis, Jellason Jessica","doi":"10.9734/sajrm/2022/v14i1262","DOIUrl":null,"url":null,"abstract":"The Bacteria Associated with cockroaches collected from food centre’s and students cafeteria of Taraba State Polytechnic Jalingo main Campus were investigated in this study between April to May 2016. A total of thirty-seven (37) adult cockroaches were collected using a sticky trap (1.40 cm x 1.50 cm). The samples collected were immediately transported to the laboratory in a conical flask. A ten (10) Told serial dilution was done. The media used were Eosine methylene blue, Salmonella Shigella agar, Nutrient agar, and MacConkey agar. Pour plate techniques was used to culture and incubation was done for 24 hours at 370c. Observable discrete colonies were identified, gram stained and subcultured using the streak plate technique of inoculation. The isolates were identified using standard techniques. The microorganisms isolated were Staphylococcus aureus, Salmonella typhi, Escherichia coli, Klebsietla species and Bacillus species. This study revealed that cockroaches can pose a public health effect. Therefore, routine sanitation and personal hygiene is strongly advocated as some of these isolates are pathogenic.","PeriodicalId":21776,"journal":{"name":"South Asian Journal of Research in Microbiology","volume":"64 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Bacteria Associated with Cockraches Collected From Some Selected Food Centres within Jalingo Metropolis\",\"authors\":\"Luka Larubi, A. Francis, Jellason Jessica\",\"doi\":\"10.9734/sajrm/2022/v14i1262\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The Bacteria Associated with cockroaches collected from food centre’s and students cafeteria of Taraba State Polytechnic Jalingo main Campus were investigated in this study between April to May 2016. A total of thirty-seven (37) adult cockroaches were collected using a sticky trap (1.40 cm x 1.50 cm). The samples collected were immediately transported to the laboratory in a conical flask. A ten (10) Told serial dilution was done. The media used were Eosine methylene blue, Salmonella Shigella agar, Nutrient agar, and MacConkey agar. Pour plate techniques was used to culture and incubation was done for 24 hours at 370c. Observable discrete colonies were identified, gram stained and subcultured using the streak plate technique of inoculation. The isolates were identified using standard techniques. The microorganisms isolated were Staphylococcus aureus, Salmonella typhi, Escherichia coli, Klebsietla species and Bacillus species. This study revealed that cockroaches can pose a public health effect. Therefore, routine sanitation and personal hygiene is strongly advocated as some of these isolates are pathogenic.\",\"PeriodicalId\":21776,\"journal\":{\"name\":\"South Asian Journal of Research in Microbiology\",\"volume\":\"64 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-12-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"South Asian Journal of Research in Microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.9734/sajrm/2022/v14i1262\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"South Asian Journal of Research in Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.9734/sajrm/2022/v14i1262","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
本研究于2016年4月至5月在塔拉巴州立理工学院Jalingo主校区的食品中心和学生食堂收集了与蟑螂相关的细菌。采用粘捕器(1.40 cm × 1.50 cm)捕获成虫37只。采集的样品立即用锥形烧瓶运送到实验室。进行10(10)次连续稀释。培养基为伊红亚甲基蓝、志贺氏沙门氏菌琼脂、营养琼脂和麦康基琼脂。采用倾板技术培养,370c孵育24小时。利用接种条纹板技术鉴定可观察到的离散菌落,进行革兰氏染色和传代培养。采用标准技术对分离物进行鉴定。分离到的微生物有金黄色葡萄球菌、伤寒沙门氏菌、大肠杆菌、克雷伯氏菌和芽孢杆菌。这项研究表明,蟑螂会对公众健康造成影响。因此,日常卫生和个人卫生是强烈提倡的,因为其中一些分离株是致病的。
Bacteria Associated with Cockraches Collected From Some Selected Food Centres within Jalingo Metropolis
The Bacteria Associated with cockroaches collected from food centre’s and students cafeteria of Taraba State Polytechnic Jalingo main Campus were investigated in this study between April to May 2016. A total of thirty-seven (37) adult cockroaches were collected using a sticky trap (1.40 cm x 1.50 cm). The samples collected were immediately transported to the laboratory in a conical flask. A ten (10) Told serial dilution was done. The media used were Eosine methylene blue, Salmonella Shigella agar, Nutrient agar, and MacConkey agar. Pour plate techniques was used to culture and incubation was done for 24 hours at 370c. Observable discrete colonies were identified, gram stained and subcultured using the streak plate technique of inoculation. The isolates were identified using standard techniques. The microorganisms isolated were Staphylococcus aureus, Salmonella typhi, Escherichia coli, Klebsietla species and Bacillus species. This study revealed that cockroaches can pose a public health effect. Therefore, routine sanitation and personal hygiene is strongly advocated as some of these isolates are pathogenic.