乳球蛋白A和β在pH值函数下锥虫水解的动力学比较研究

Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects Pub Date : 1964-10-23 DOI:10.1016/0926-6569(64)90274-3

Monique Monnot, Jeannine Yon

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引用次数: 5

摘要

在不同的pH值下观察乳球蛋白A和B的胰蛋白酶水解。动力学含量随pH值的变化，给我们提供了一些反应机理的信息。在中间复合物形成过程中，与乳球蛋白(A型或B型)结合的酶诱导底物分子的坦福德转化，使异常羧基消失，从而促进其电离。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Étude cinétique comparée de l'hydrolyse trypsique des lactoglobulines A et B fonction du pH

The tryptic hydrolysis of lactoglobulins A and B is followed for different pH values. The variations of the kinetic contants according to the pH, give us some information on the reaction mechanism. During the intermediary complex formation, the enzyme associating with the lactoglobulin (A or B form) induces the Tanford transformation of the substrate molecule with demasking of the anomalous carboxylic groups thereby facilitating their ionization.

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