{"title":"密螺旋体denticola fliQ区的分子特征","authors":"L. Stamm, H. Bergen","doi":"10.3109/10425170109084475","DOIUrl":null,"url":null,"abstract":"A Treponema denticola 4.2 kb DNA region containing four complete genes (orf1, fliQ, fliR, and flhB) and a truncated gene (flhA1) was sequenced and analyzed. The deduced amino acid sequences of FliQ, FliR, FlhB and FlhA' have significant homology with bacterial proteins associated with the flagellar export apparatus, whereas the deduced amino acid sequence of Orfl has homology with an E. coli alcohol dehydrogenase. A putative α70-like promoter was identified upstream of fliQ. RT-PCR analysis indicated that fliQ, fliR, flhB and flhA1 are co-transcribed independently of orfl, suggesting that the motility-associated genes are components of an operon. The location of the T. denticola fliQ-flhA1 genes differs from that of the corresponding T. pallidum and Borrelia burgdorferi genes which are present in the large fla or flgB flagellar operons, respectively.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"2 1","pages":"463 - 467"},"PeriodicalIF":0.0000,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Molecular Characterization of the Treponema denticola fliQ Region\",\"authors\":\"L. Stamm, H. Bergen\",\"doi\":\"10.3109/10425170109084475\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A Treponema denticola 4.2 kb DNA region containing four complete genes (orf1, fliQ, fliR, and flhB) and a truncated gene (flhA1) was sequenced and analyzed. The deduced amino acid sequences of FliQ, FliR, FlhB and FlhA' have significant homology with bacterial proteins associated with the flagellar export apparatus, whereas the deduced amino acid sequence of Orfl has homology with an E. coli alcohol dehydrogenase. A putative α70-like promoter was identified upstream of fliQ. RT-PCR analysis indicated that fliQ, fliR, flhB and flhA1 are co-transcribed independently of orfl, suggesting that the motility-associated genes are components of an operon. The location of the T. denticola fliQ-flhA1 genes differs from that of the corresponding T. pallidum and Borrelia burgdorferi genes which are present in the large fla or flgB flagellar operons, respectively.\",\"PeriodicalId\":11381,\"journal\":{\"name\":\"DNA Sequence\",\"volume\":\"2 1\",\"pages\":\"463 - 467\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2001-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"DNA Sequence\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3109/10425170109084475\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"DNA Sequence","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3109/10425170109084475","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
摘要
对齿状密螺旋体4.2 kb DNA区包含4个完整基因(orf1、fliQ、fliR和flhB)和一个截断基因(flhA1)进行测序和分析。flq、FliR、FlhB和FlhA'的氨基酸序列与鞭毛输出器相关的细菌蛋白具有显著的同源性，而Orfl的氨基酸序列与大肠杆菌乙醇脱氢酶具有显著的同源性。在fliQ上游发现了一个推测的α70样启动子。RT-PCR分析表明，fliQ、fliR、flhB和flhA1独立于orfl共转录，提示运动相关基因是一个操纵子的组成部分。denticola T. fliQ-flhA1基因与对应的T. pallidum和Borrelia burgdorferi基因的位置不同，后者分别存在于大鞭毛操纵子或flgB鞭毛操纵子中。
Molecular Characterization of the Treponema denticola fliQ Region
A Treponema denticola 4.2 kb DNA region containing four complete genes (orf1, fliQ, fliR, and flhB) and a truncated gene (flhA1) was sequenced and analyzed. The deduced amino acid sequences of FliQ, FliR, FlhB and FlhA' have significant homology with bacterial proteins associated with the flagellar export apparatus, whereas the deduced amino acid sequence of Orfl has homology with an E. coli alcohol dehydrogenase. A putative α70-like promoter was identified upstream of fliQ. RT-PCR analysis indicated that fliQ, fliR, flhB and flhA1 are co-transcribed independently of orfl, suggesting that the motility-associated genes are components of an operon. The location of the T. denticola fliQ-flhA1 genes differs from that of the corresponding T. pallidum and Borrelia burgdorferi genes which are present in the large fla or flgB flagellar operons, respectively.
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