Spectrophotometric Determination and Cloud Point Extraction of Cefixime Drugs in Pure form and Pharmaceutical Preparation

: A simple, rapid, accurate, sensitive and eco friendly method has been developed for the quantitative determination of cefixime(CFX) in pure form and pharmaceutical preparations by using a combination of cloud point extraction with UV-Visible absorption spectrophotometric method. Analytical applications of complexation with metal ions by reacting cefixime (CFX) with copper(II) and iron(III) to form chelate complexes under limited experimental conditions. The method based to dissolved CFX in 0.1 M NaOH, 10% (v/v) triton x-114 and mixed with (1000 µ gmL -1 ) copper(II) or (1000 µ gmL -1 ) iron(III). The formation of CFX-Cu(II) complex at pH 13 and wavelength 827 while the complex of CFX-Fe(III) was formatted at pH 11 and wavelength at 439 nm. The complexes of CFX-Cu(II) and CFX-Fe(III) obey Beer’s Law in the range 10-130 and 10-160 µ g/mL respectively. LOD and LOQ values for these complexes were 1.6906 µ g/mL and 5.6355 µ g/mL and LOQ values were 1.58655 µ g/mL and 5.2887 µ g/mL respectively. Method was validated and successfully applied to drug formulations like syrup infusion marketed in Amman and cefixime capsules marketed in Iraq. The results of analysis have been validated statistically by recovery studies and were found satisfactory.