Invitro anti-inflammatory activity of newly synthesized mannich base 2-[(1,3-benzothiazol-2-ylamino)methyl]-5-methyl-2,4-dihydro-3H-pyrazol-3-one and its derivatives

The present study was aimed to synthesize a series 2-[(1,3-benzothiazol-2-ylamino)methyl]-5-methyl-2,4-dihydro-3 H -pyrazol-3-one (3 a-l) and to evaluate their in-vitro anti-inflammatory activity. The starting material (1a-l) was prepared by the application of cyclization reaction. In-vitro anti-inflammatory activity of synthesized compounds [3a-l] was evaluated using inhibition of bovine serum albumin denaturation method. 3c and 3l have shown significant in-vitro anti-inflammatory activity. The findings of present study clearly demonstrate that chloro functional group possess inhibition of bovine serum albumin denaturation capacity and has in-vitro anti-inflammatory activity. However methyl, methoxy and dimethyl derivatives show mild to moderate in-vitro anti-inflammatory activity.