The effect of phytase on the liver tissue after infection with Entamoeba histolytica in adult albino male rat

The objective of current study was designed to show the activity of phytase enzymes against E. histolytica and its effectiveness to recovery liver tissue after infection. 24 adult male rats (with any diseases) were used and divided into four groups (6 rats in each group); control group feeding on standard pellet diet, second group administrated with E. histolyticaat dose 10 3 cyst/ ml. Third group: administrated with (10 3 cyst/ ml) E. histolyticaand feeding with special diet content (25mg/kg) phytase for four weeks. Fourth group: administrated with (10 3 cyst/ ml) E. histolyticaand feeding with special diet content (50mg/kg) phytasefor four weeks. About oxidative stress, Levels of Malondialdehyde (MDA) and catalase (antioxidant enzyme) show high significant changes (P < 0.05) in an infected (with E. histolytica) group compare with control. After using phytase, levels of MDA and catalase in third and four groups show non-significant changes (P < 0.05) compared with control. The sections of infected group degeneration and necrosis of hepatocytes, with picknotic nuclei of some hepatocytes. The section of (25mg/kg) phytase group shows show thickening wall of some central veins. The sections of (50mg/kg) phytase group show no difference between this group and control group. It was concluded that phytase have been potential role against E. histolytica.