Co-administration of oleic and docosahexaenoic acids enhances glucose uptake rather than lipolysis in mature 3T3-L1 adipocytes cell culture

This study investigated the effect of different types of long-chain fatty acids and their combination on the triglyceride accumulation, glucose utilisation, and lipolysis in already obese adipocytes. 3T3-L1 MBX cells were first differentiated into mature adipocytes using adipogenic inducers (3-isobutyl-1-methylxanthine, dexamethasone, indomethacin, insulin, and high glucose), then 100 µM 0.1% ethanol extracts of palmitic (PA), oleic (OA), or docosahexaenoic acid (DHA) were applied for nine days. Unsaturated fatty acids decreased the intracellular lipid accumulation while maintaining glucose utilisation levels. However, unlike OA, self-administration of DHA only intensified lipolysis by 25% vs induced untreated control (IC), which may have a direct detrimental impact on the whole body’s metabolic state. DHA applied in equal proportion with PA elevated triglyceride accumulation by 10% compared to IC, but applied with OA, enhanced glucose uptake without any significant changes in the lipogenic drive and the lipolytic rate, suggesting that this unsaturated fatty acids combination may offer a considerable advantage in amelioration of obesity-related disorders.