筛查尼日利亚贝努埃州马库尔迪屠宰场环境中具有公共卫生重要性的细菌

N. NseabasiMaina, E. G. Vinkings, I. Bassey, A. Unimke, L. O. Abulawor
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引用次数: 1

摘要

该研究调查了一个大型屠宰场的细菌含量,该屠宰场距离贝努埃河沿岸人口稠密的耕地约300米。在本研究中,对样品中的细菌细胞进行计数后,出水和土壤样品的平均细菌细胞计数分别为9.4 × 105和7.3 × 105。从两个样本中分离的细菌包括;大肠杆菌、链球菌、沙门氏菌、假单胞菌、志贺氏菌、肠杆菌、葡萄球菌、芽孢杆菌、布鲁氏菌、变形杆菌、微球菌等。污水中大肠杆菌和土壤中大肠杆菌的发生率分别为18.53%和16.16%,土壤样品中变形杆菌和布鲁氏菌的发生率分别为9.59%和1.39%。使用17种抗生素筛选盘(Optun Nig.)进行抗生素敏感性筛选,即:塔利维德(10µg)、佩弗拉辛(10µg)、阿古门汀(30µg)、庆大霉素(10µg)、链霉素(30µg)、头孢雷(10µg)、萘利地酸(30µg)、环丙沙星(10µg)、诺氟沙星(10µg)、利福平(10µg)、红霉素(10µg)、氯霉素(10µg)、氨苄氯(30µg)、左氧氟沙星(10µg)。在分离株中观察到明显的抗性水平。大肠杆菌对培氟平敏感,志贺氏菌对扩增素敏感,肠球菌和芽孢杆菌分别对环丙沙星和链霉素敏感。
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Screening the Milieu of an abattoir for Bacteria of Public health importance in Makurdi, Benue State, Nigeria
The study investigated the bacteriological content in a major abattoir located approximately 300 meters from a densely populated and cultivated area along the Benue River. Enumeration of bacterial cells from samples in the study yielded relatively high mean count of 9.4 x 105 and 7.3 x 105 from effluent and soil samples respectively. Bacteria isolated from both samples included; Escherichia coli, Streptococcus sp, Salmonella sp, Pseudomonas sp, Shigellasp, Enterobacter sp, Staphylococcus sp, Bacillus sp, Brucella sp, Proteus sp, Micrococcus sp etc. Escherichia coli recorded an occurrence of 18.53% in effluents and 16.16% in soil while Proteus species and Brucella sp had an occurrence of 9.59% and 1.39% respectively in soil samples. Antibiotic sensitivity screening using seveenteen (17) antibiotics disc (Optun Nig.) viz: Tarivid (10 µg), peflacine (10 µg), Agumentin (30 µg), Gentamycin (10 µg), Streptomycin (30 µg), Ceporex (10 µg), Nalidixic acid (30 µg), Ciprofloxacin (10 µg), Norfloxacin (10 µg), Rifampicin (µg), Erythromycin (µg), Chloramphenicol (µg), Ampiclox (30 µg), Levofloxacin (10µg). A marked level of resistance was observed among the isolates. However, Escherichia coli indicated sensitivity to peflacine, Shigellasp indicated sensitivity to augmentin, Enterococcus and Bacillus species indicated sensitivity to ciprofloxacin and streptomycin respectively.
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