顿河畔罗斯托夫地表水库分离的条件致病性肠杆菌的生物多样性和抗生素耐药性

A. Trishina, E. Bereznyak, I. Simonova, L. M. Verkina, A. Bereznyak, M. V. Poleeva
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引用次数: 4

摘要

的目标。研究2014 - 2016年从顿河畔罗斯托夫地表水库分离的肠杆菌科机会微生物的生物多样性及对抗菌制剂(ABP)的敏感性/耐药性。材料和方法。利用MALDI Biotyper程序-仪器单元进行复杂的生化试验,确定肠杆菌属和种成员。采用串联稀释法测定对ABP的敏感性。结果:共分离到机会致病性肠杆菌468株。鉴定出微生物20属33种。以克雷伯菌(37.0%)、柠檬酸杆菌(8.0%)为主。2014年分离株对ABP敏感的为8.5%,2015年为5.2%;2016年未检出对ABP敏感的菌株。在过去3年中,多耐药菌株的数量有所增加:2014年为40.7%,2015年为60.1%。2016年为80%。头孢曲松、庆大霉素和环丙沙星对分离的肠杆菌有较高的抑菌活性。最高的耐药是氨苄西林,萘啶酸,硝基呋喃,复方新诺明。结论。获得的数据允许评估从地表水库分离的肠杆菌多耐药菌株的增加动态。
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BIODIVERSITY AND ANTIBIOTICS RESISTANCE OF OPPORTUNISTIC PATHOGENIC ENTEROBACTERIA ISOLATED FROM SURFACE RESERVOIRS OF ROSTOV-ON-DON
Aim. Study biodiversity, sensitivity/resistance to antibacterial preparation (ABP) of opportunistic microorganisms of Enterobacteriaceae family isolated from surface reservoirs of Rostov-on-Don in 2014 - 2016. Materials and methods. Determination of genus and species membership of enterobacteria was carried out by the results of a complex of biochemical tests using MALDI Biotyper program-apparatus unit. Sensitivity to ABP was determined by serial dilution method. Results. 468 strains of opportunistic and pathogenic enterobacteria were isolated during the study. 20 genera, 33 species of microorganisms were identified. (37.0%), Klebsiella (13.2%), Citrobacter (8.0%) predominated. 8.5% of the strains isolated in 2014 were sensitive to ABP, 5.2% - in 2015; strains sensitive to ABP were not detected in 2016. The number of polyresistant isolates has increased during the last 3 years: 40.7% strains in 2014,60.1 % in 2015. 80,0% in 2016. Ceftriaxone, gentamycin and ciprofloxacin had high activity against the isolated enterobacteria. The highest resistance was documented against ampicillin, nalidixic acid, nitro-furane, co-trimoxazole. Conclusion. The data obtained allow to evaluate the dynamics of increase of polyresistant strains of enterobacteria isolated from surface reservoirs.
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来源期刊
Zhurnal mikrobiologii, epidemiologii, i immunobiologii
Zhurnal mikrobiologii, epidemiologii, i immunobiologii Immunology and Microbiology-Immunology and Microbiology (miscellaneous)
CiteScore
1.40
自引率
0.00%
发文量
51
审稿时长
8 weeks
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