Li Liu, Nanbiao Long, Juan Zhou, Manxue Liu, Shaobo He, Wuying Chu
{"title":"水产品中恩诺沙星和环丙沙星的方法验证及测量不确定度评定。","authors":"Li Liu, Nanbiao Long, Juan Zhou, Manxue Liu, Shaobo He, Wuying Chu","doi":"10.1155/2023/5554877","DOIUrl":null,"url":null,"abstract":"<p><p>This study aimed to investigate a detection method of enrofloxacin and ciprofloxacin to be avail for strictly supervising the quality and safety of aquatic products. The results displayed that the optimal extraction conditions for enrofloxacin and ciprofloxacin were the following five aspects: 15 g dosages of Na<sub>2</sub>SO<sub>4</sub> to dehydrate, 8‰ of acetonitrile and 50% hydrochloric acid to deproteinization, 2 mL dosages of n-hexane to degrease, 10 min of ultrasonic time, and 20 min of extraction (stand) time. Meanwhile, it was also obtained for the optimal detection performance indexes of the recovery, precision, and accuracy from the tests of <i>shrimp</i>, <i>grass carp</i>, and <i>tilapia</i>. In particular, the expanded uncertainties were 2.8601 and 0.8613, and the factors of both the calibration curves (<i>U</i><sub>rel(C)</sub>) and the analysis of the experiment (<i>U</i><sub>rel(E)</sub>) were the two MU main contributors for enrofloxacin and ciprofloxacin together with the results above 40%. Consequently, the developed novel method was suited for the determination of the enrofloxacin and ciprofloxacin residues in aquatic products and would contribute to reinforce in supervision and inspection of the quality and safety of aquatic products.</p>","PeriodicalId":13888,"journal":{"name":"International Journal of Analytical Chemistry","volume":"2023 ","pages":"5554877"},"PeriodicalIF":1.5000,"publicationDate":"2023-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10640130/pdf/","citationCount":"0","resultStr":"{\"title\":\"Method Validation and Measurement Uncertainty (MU) Evaluation on Enrofloxacin and Ciprofloxacin in the Aquatic Products.\",\"authors\":\"Li Liu, Nanbiao Long, Juan Zhou, Manxue Liu, Shaobo He, Wuying Chu\",\"doi\":\"10.1155/2023/5554877\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study aimed to investigate a detection method of enrofloxacin and ciprofloxacin to be avail for strictly supervising the quality and safety of aquatic products. The results displayed that the optimal extraction conditions for enrofloxacin and ciprofloxacin were the following five aspects: 15 g dosages of Na<sub>2</sub>SO<sub>4</sub> to dehydrate, 8‰ of acetonitrile and 50% hydrochloric acid to deproteinization, 2 mL dosages of n-hexane to degrease, 10 min of ultrasonic time, and 20 min of extraction (stand) time. Meanwhile, it was also obtained for the optimal detection performance indexes of the recovery, precision, and accuracy from the tests of <i>shrimp</i>, <i>grass carp</i>, and <i>tilapia</i>. In particular, the expanded uncertainties were 2.8601 and 0.8613, and the factors of both the calibration curves (<i>U</i><sub>rel(C)</sub>) and the analysis of the experiment (<i>U</i><sub>rel(E)</sub>) were the two MU main contributors for enrofloxacin and ciprofloxacin together with the results above 40%. Consequently, the developed novel method was suited for the determination of the enrofloxacin and ciprofloxacin residues in aquatic products and would contribute to reinforce in supervision and inspection of the quality and safety of aquatic products.</p>\",\"PeriodicalId\":13888,\"journal\":{\"name\":\"International Journal of Analytical Chemistry\",\"volume\":\"2023 \",\"pages\":\"5554877\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2023-11-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10640130/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Analytical Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1155/2023/5554877\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Analytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1155/2023/5554877","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Method Validation and Measurement Uncertainty (MU) Evaluation on Enrofloxacin and Ciprofloxacin in the Aquatic Products.
This study aimed to investigate a detection method of enrofloxacin and ciprofloxacin to be avail for strictly supervising the quality and safety of aquatic products. The results displayed that the optimal extraction conditions for enrofloxacin and ciprofloxacin were the following five aspects: 15 g dosages of Na2SO4 to dehydrate, 8‰ of acetonitrile and 50% hydrochloric acid to deproteinization, 2 mL dosages of n-hexane to degrease, 10 min of ultrasonic time, and 20 min of extraction (stand) time. Meanwhile, it was also obtained for the optimal detection performance indexes of the recovery, precision, and accuracy from the tests of shrimp, grass carp, and tilapia. In particular, the expanded uncertainties were 2.8601 and 0.8613, and the factors of both the calibration curves (Urel(C)) and the analysis of the experiment (Urel(E)) were the two MU main contributors for enrofloxacin and ciprofloxacin together with the results above 40%. Consequently, the developed novel method was suited for the determination of the enrofloxacin and ciprofloxacin residues in aquatic products and would contribute to reinforce in supervision and inspection of the quality and safety of aquatic products.
期刊介绍:
International Journal of Analytical Chemistry publishes original research articles that report new experimental results and methods, especially in relation to important analytes, difficult matrices, and topical samples. Investigations may be fundamental, or else related to specific applications; examples being biological, environmental and food testing, and analysis in chemical synthesis and materials processing.
As well as original research, the International Journal of Analytical Chemistry also publishes focused review articles that examine the state of the art, identify emerging trends, and suggest future directions for developing fields.