T. Ootaki, A. Miyazaki, J. Fukui, Yuji Kimura, Seiichi Umemura, R. Okazaki, M. Jayaram
{"title":"一种外源基因导入黑藻的高效方法","authors":"T. Ootaki, A. Miyazaki, J. Fukui, Yuji Kimura, Seiichi Umemura, R. Okazaki, M. Jayaram","doi":"10.1266/JJG.66.189","DOIUrl":null,"url":null,"abstract":"An efficient method is described for obtaining transformants in Phycomyces by microinjection of plasmid DNA carrying the kanamycin resistance gene from Tn903 into a young sporangium. Approximately 9% of the colonies, germinated from the spores which were developed in the sporangium, were resistant to G418 when a 36 ng sample of the transforming plasmid DNA was injected into a sporangium. The transformation efficiency (transfomants/total colonies) was about 350 times higher than that (transfomants/total protoplasts) obtained with the ordinary protoplast method.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"29 1","pages":"189-195"},"PeriodicalIF":0.0000,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"11","resultStr":"{\"title\":\"A high efficient method for introduction of exogenous genes into Phycomyces blakesleeanus\",\"authors\":\"T. Ootaki, A. Miyazaki, J. Fukui, Yuji Kimura, Seiichi Umemura, R. Okazaki, M. Jayaram\",\"doi\":\"10.1266/JJG.66.189\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"An efficient method is described for obtaining transformants in Phycomyces by microinjection of plasmid DNA carrying the kanamycin resistance gene from Tn903 into a young sporangium. Approximately 9% of the colonies, germinated from the spores which were developed in the sporangium, were resistant to G418 when a 36 ng sample of the transforming plasmid DNA was injected into a sporangium. The transformation efficiency (transfomants/total colonies) was about 350 times higher than that (transfomants/total protoplasts) obtained with the ordinary protoplast method.\",\"PeriodicalId\":22578,\"journal\":{\"name\":\"The Japanese Journal of Genetics\",\"volume\":\"29 1\",\"pages\":\"189-195\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1991-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"11\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Japanese Journal of Genetics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1266/JJG.66.189\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Japanese Journal of Genetics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1266/JJG.66.189","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
A high efficient method for introduction of exogenous genes into Phycomyces blakesleeanus
An efficient method is described for obtaining transformants in Phycomyces by microinjection of plasmid DNA carrying the kanamycin resistance gene from Tn903 into a young sporangium. Approximately 9% of the colonies, germinated from the spores which were developed in the sporangium, were resistant to G418 when a 36 ng sample of the transforming plasmid DNA was injected into a sporangium. The transformation efficiency (transfomants/total colonies) was about 350 times higher than that (transfomants/total protoplasts) obtained with the ordinary protoplast method.
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