咖啡叶次生代谢物的分离)及其抑菌活性试验

D. Roza, Nova Betria Sinaga, Christesya Ambarita
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引用次数: 0

摘要

咖啡寄生虫叶(Loranthus parasiticus (L.))山茱萸(Merr)是一种药用植物,常见于亚热带或热带地区。本研究旨在通过圆盘扩散法和微量稀释法分离咖啡叶甲醇提取物的次生代谢产物,并对金黄色葡萄球菌、变形葡萄球菌和葡萄球菌进行抑菌活性研究。对金黄色葡萄球菌、变形葡萄球菌和绿葡萄球菌的抑菌活性分别为7.5 mm;7.9毫米;8 mm即表明对甲醇提取物细菌生长的抑制能力为1%的咖啡叶属于中等范畴。微量稀释法测定KHM对金黄色葡萄球菌、变形葡萄球菌和葡萄球菌的抑菌值均为5000 μg/mL。同时,对金黄色葡萄球菌的MBC值>5000 g/Ml,对变形葡萄球菌的MBC值>5000 g/Ml,对青绿葡萄球菌的MBC值为5000 g/Ml,说明咖啡寄生虫叶甲醇提取物仅具有抑制作用。二级代谢物化合物的分离采用液相真空色谱和重力柱色谱分离,并进一步采用气相色谱-质谱仪进行表征。咖啡寄生虫(Loranthus parasiticus, L.)叶片甲醇提取物中次生代谢物的分离结果Merr.)表明咖啡寄生虫的叶子含有任意9种化合物。
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Isolation of secondary metabolite compounds of Coffee Benalu leaves (Loranthus parasiticus (L.) Merr.) and its antibacterial activity test
Coffee parasite leaves (Loranthus parasiticus (L.) Merr) is one of the plants that can be used as a medicinal material commonly obtained in various subtropical regions or tropical regions. This study aims to isolation of secondary metabolite compounds from methanol extract of coffee leaves and antibacterial activity by of disc diffusion and microdilution methods against S. aureus bacteria, S. mutans, and S. viridians. The result of antibacterial activity against S. aureus bacteria, S. mutans, and S. viridians respectively is 7.5 mm; 7.9 mm; and 8 mm which indicates that the ability to inhibit the growth of methanol extract bacteria 1% coffee leaves belongs to the medium category. The results of the microdilution method in determining the value of KHM against bacteria S. Aureus, S. Mutans, and S. Viridians are equal to the KHM value of 5000 μg/mL. Meanwhile, the MBC value for S. aureus was >5000 g/Ml, for S. mutans was >5000 g/mL and for S. viridians it was 5000 g/mL, indicating that the methanol extract of the coffee parasite leaves was only an inhibitor. Isolation of secondary metabolite compounds is carried out by fractionation using Liquid Vacuum Chromatography and Gravitational Column Chromatography which are further characterized using the GC-MS instrument. The results of the isolation of secondary metabolites from the methanol extract of the leaves of the coffee parasite (Loranthus parasiticus (L.) Merr.) showed that the leaves of the coffee parasite contained any 9 compounds.
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