寄生虫灭病毒活性:对陆地上致病性生物废物管理的影响

Lakshmi Amaravadi , Michael S. Bisesi , Robert F. Bozarth
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引用次数: 11

摘要

以豇豆花叶病毒(CPMV)和烟草花叶病毒(TMV)为模型剂,对已知含有杀菌酶的蚯蚓进行了杀病毒活性试验。用含病毒悬浮液的纤维素喂蚯蚓,用酶联免疫吸附试验(ELISA)和局部病变试验分析蚯蚓排泄物中病毒蛋白的结构完整性。饲养实验的观察表明,这两种病毒的传染性都大大降低。将病毒悬浮液与蚯蚓酶提取物一起孵育,用局部损伤实验分析其杀病毒活性。观察到的两种病毒传染性的降低表明,恶臭杆菌可能具有杀病毒酶系统,因此可能有助于病原病毒的灭活,这可能与污水污泥和牲畜粪便的土地施用有关。
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Vermial virucidal activity: Implications for management of pathogenic biological wastes on land

The earthworm Eisenia fetida, known to contain bactericidal enzymes, was tested for virucidal activity using Cowpea mosaic virus (CPMV) and Tobacco mosaic virus (TMV) as model agents. Earthworms were fed cellulose saturated with a virus suspension and their excreted castings were analyzed for structurally intact virus protein using enzyme-linked immunosorbant assay (ELISA) and virus infectivity by local lesion assays. Observations of the feeding experiments indicated a considerable reduction in the infectivity of both viruses. Virucidal activity was also observed when virus suspensions were incubated with the earthworm enzyme extract and analyzed by local lesion assay. The observed reductions in the infectivity of both viruses suggest that E. fetida may possess a virucidal enzyme system and, accordingly, may contribute to the inactivation of pathogenic viruses potentially associated with land application of sewage sludges and livestock manures.

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