苏拉威西植物苏拉威西天然植物

R. Pitopang, E. Lestari, Puti Andalusia Sarigando Banilai, W. Harso
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引用次数: 0

摘要

题为“苏拉威西岛特有植物Etlingera sublimata Poulsen (Zingiberaceae)的植物化学筛选和抗菌活性”的研究于2020年1月至2020年6月在Tadulako大学数学与自然科学学院生物系植物生物系统学实验室和生物技术实验室进行。本文采集了位于Poso Regency洛尔乌塔拉区塞多阿村附近的洛尔林杜国家公园(LLNP)的高山森林中,研究了洛尔林杜植物的次生代谢产物,并测定了洛尔乌塔拉叶提取物对沙门菌的抑菌活性。以96%乙醇为溶剂,采用浸渍法提取植物样品。采用琼脂孔扩散法测定活性菌活性。试验采用完全随机设计(CRD),采用7种不同浓度(15、25、50、75、85%)的野牡丹提取物进行设计。用营养琼脂管在双重蒸馏水中制备提取液和标准药物。以2%氯霉素为标准药为阳性对照,无菌试纸为阴性对照。37℃孵育18 ~ 24 h后,测定圆盘周围细菌和酵母生长的抑制带。结果表明,该茎中含有生物碱、黄酮类、萜类、单宁和皂苷。根茎含有生物碱、黄酮类、萜类、单宁和皂苷,花含有生物碱、萜类和单宁,叶含有单宁和皂苷化合物。叶提取物对致病性伤寒沙门菌的生长有抑制作用,有效浓度为50%,平均抑制面积为2.67 mm。
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FITOKIMIA, DAN AKTIFITAS ANTIBAKTERI DARI Etlingera sublimata Poulsen (ZINGIBERACEAE), TUMBUHAN ENDEMIK SULAWESI
The research entitled "Phytochemical Screening and antibacterial activity  of Etlingera sublimata Poulsen (Zingiberaceae), an Endemic Sulawesi of Plants  was conducted from January 2020 to June 2020, at the Plant Biosystematics Laboratory and Biotechnology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Tadulako University. Samples of E. sublimata  were collected  from the mountane forests of the Lore Lindu National Park (LLNP), arround Sedoa village, Lore Utara District, Poso Regency The objectives of the research were to analyze the   secondary metabolite compounds in E. sublimata plants and to determine the antibacterial activity of E. sublimata leaves extract in againts Salmonella thypii bacteria. Plant samples were extracted by maceration methods with 96% ethanol as a solvent. Actibacterial activity was tested by using agar well diffusion  methods. The experiment was designed by Completely Randomized Design (CRD) with seven different concentration of E. sublimata  extract (15, 25, 50, 75 and 85%). Extract and standard drugs were prepared in double-distilated water using Nutrient Agar tubes.  2% Chloramphenicol as standard drugs was  used as a  positive control and sterile aquadest was used as a negative control. The inhibition zone of bacteria and yeast growth around the disk was measured after 18 to 24 h incubation at 37°C.   The results showed that the stem contains alkaloids, flavonoids, terpenoids, tannins and saponins. The rhizome containts alkaloid, flavonoids, terpenoids, tannins and saponins, the flowers containts alkaloid, terpenoids and tannins, while the leaves containts tannin and saponin compounds. Leaves extract of E.sublimata has inhibition activity on the cell growth a pathogenic Salmonella thypi bacteria that the effective extract concentration was 50% with an average inhibition zone  ​​2.67 mm.
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