{"title":"发布信息TOC","authors":"","doi":"10.1002/cpmb.94","DOIUrl":null,"url":null,"abstract":"<p><b>Cover</b>: Related to Laughery and Wyrick (https://doi.org/10.1002/cpmb.110), The cover image shows the experimental strategy for simple CRISPR genome editing in yeast. The central image depicts how Cas9-induced DNA breaks can be used to generate marker-free genome edits in yeast by homologous recombination (HR) with a repair template. The outer circle depicts the organization of a Cas9/sgRNA expression vector used for simple CRISPR/Cas9 genome editing in yeast.\n\n <figure>\n <div><picture>\n <source></source></picture><p></p>\n </div>\n </figure></p>","PeriodicalId":10734,"journal":{"name":"Current Protocols in Molecular Biology","volume":"129 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmb.94","citationCount":"0","resultStr":"{\"title\":\"Issue Information TOC\",\"authors\":\"\",\"doi\":\"10.1002/cpmb.94\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><b>Cover</b>: Related to Laughery and Wyrick (https://doi.org/10.1002/cpmb.110), The cover image shows the experimental strategy for simple CRISPR genome editing in yeast. The central image depicts how Cas9-induced DNA breaks can be used to generate marker-free genome edits in yeast by homologous recombination (HR) with a repair template. The outer circle depicts the organization of a Cas9/sgRNA expression vector used for simple CRISPR/Cas9 genome editing in yeast.\\n\\n <figure>\\n <div><picture>\\n <source></source></picture><p></p>\\n </div>\\n </figure></p>\",\"PeriodicalId\":10734,\"journal\":{\"name\":\"Current Protocols in Molecular Biology\",\"volume\":\"129 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-12-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1002/cpmb.94\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Protocols in Molecular Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/cpmb.94\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Molecular Biology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cpmb.94","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
Cover: Related to Laughery and Wyrick (https://doi.org/10.1002/cpmb.110), The cover image shows the experimental strategy for simple CRISPR genome editing in yeast. The central image depicts how Cas9-induced DNA breaks can be used to generate marker-free genome edits in yeast by homologous recombination (HR) with a repair template. The outer circle depicts the organization of a Cas9/sgRNA expression vector used for simple CRISPR/Cas9 genome editing in yeast.
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