绿茶水和甲醇叶提取物对病原菌的抑菌活性研究

Shahlaa M. Saleh, B. Saleh, A. EnasS.Bahjat
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引用次数: 1

摘要

研究了茶树水提液和甲醇叶提液对病原菌的抑菌活性。采用纸片扩散试验法测定金黄色葡萄球菌(SR1)、金黄色葡萄球菌(SR2)、鼠伤寒沙门氏菌、大肠杆菌、铜绿假单胞菌和变形杆菌对氨苄西林、链霉素、利福平、新霉素等不同抗生素的敏感性。结果发现金黄色葡萄球菌(SR1)、金黄色葡萄球菌(SR2)、铜绿假单胞菌和鼠伤寒沙门氏菌均对氨苄西林和利福平耐药。大肠杆菌对氨苄西林、利福平和新霉素耐药,而Proteus对本研究中使用的所有抗生素均耐药。研究了不同浓度(22、02、02、02、122)mg/ml绿茶水提物和甲醇叶提物对病原菌的抑菌活性。结果表明,绿茶水和甲醇叶提取物对病原菌均有抑制作用,甲醇提取物对金黄色葡萄球菌的抑菌效果较好。
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Antimicrobial Activity of Water and Methanol Leaf Extracts of Green Tea (Camellia Sinensis) Against Some Pathogenic Bacteria
This study was carried out to investigate the antimicrobial activity of water and methanol leaf extracts of Camillia sinensis against some pathogenic bacteria. Susceptibilities of S. aureus (SR1), S. aureus (SR2), S.typhimurium, E.coli, P. aeruginosa and Proteus to different antibiotics (Ampicillin, Streptomycin, Rifampicin and Neomycin) by disc diffusion test was carried out. Results revealed that S. aureus (SR1), S. aureus (SR2), P.aeruginosa, and S.typhimurium were resistant to Ampicillin and Rifampicin. E. coli was resistant to Ampicillin, Rifampicin and Neomycin while Proteus was resistant to all antibiotics used in this study. Antimicrobial activity of water and methanol leaf extracts of green tea at different concentrations (22,02,02,02 and 122) mg/ml against pathogenic bacteria was tested. Results showed that green tea water and methanol leaf extract showed inhibitory effect against pathogenic bacteria and methanol extract exhibited better antimicrobial effect against Staphylococcus aureus.
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