白芍马来茶的生物活性成分及薄层色谱分析

Tunde Ayobami Owolabi, Dominion Osaretin, Blessing Eyinayan
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引用次数: 1

摘要

任何草药的生物/药理活性都是由其配方中使用的植物中所含的生物活性成分决定的,这些物质在生产过程中和生产过程后以及处理过程中所采用的质量控制系统很大程度上影响了这些物质。薄层色谱就是这样一种质量控制参数,它体现了各种物质之间的唯一性和均匀性,从而可以作为这些产品的身份。Pax草药疟疾治疗茶是一种用于管理和治疗疟疾症状的多草药药物。本研究评估了不同植物成分的存在,并建立了该药物(Pax Herbal Malatreat Tea)的指纹图谱(TLC),以作为生产一致性和市场独特性的质量控制检查。采用标准方法进行定性植物化学和色谱分析。定性分析发现,其主要成分为单宁、黄酮类、皂苷、甾体、还原糖和萜类;用氯仿-乙酸乙酯(9.5:05)显影后的指纹图谱在365 nm处显示出5种不同的成分,在可见光下用20%甲醇硫酸喷扫后发现有4个斑点。根据本报告,通过生物活性成分和薄层色谱图谱为药物(Pax Herbal Malatreat Tea)开发了身份证,可用于获取产品的质量和一致性。
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Bioactive composition and TLC profile data on Pax Herbal Malatreat Tea
The biological/pharmacological activities of any herbal drug are determined by the bioactive components contained in the plant(s) used in its formulation, these substances are greatly affected by the quality control system adopted during and after production processes and handling of such product. Thin Layer Chromatography is one of such quality control parameters that demonstrate uniqueness and uniformity between various substances, thus serving as an identity for such products.  Pax Herbal Malatreat tea is a polyherbal drug used in the management and treatment of malaria symptoms. This study evaluated the different phytoconstituents present and developed fingerprint profiles (TLC) for the drug (Pax Herbal Malatreat Tea) to serve as a quality control check during the production consistency and market uniqueness. Qualitative phytochemical and chromatographic analyses were carried out using standard methods. The qualitative test revealed the presence of Tannin, Flavonoid, Saponin, Steroids, Reducing sugar, and Terpenoids, and the finger-print chromatograms after development with chloroform-ethyl acetate (9.5:05) showed five distinct components at 365 nm and four spots when sprayed with 20% methanolic sulphuric acid and viewed under visible light. From this present report, an identity card has been developed for the drug (Pax Herbal Malatreat Tea) via bioactive composition and TLC profiles which can be used in accessing the quality and consistency of the product.
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