含外源基因的温和气单胞菌脂肪酶的生产与固定化

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI:10.1016/S0922-338X(98)80140-2

Saovanee Dharmsthiti, Sudaporn Luchai

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引用次数: 3

摘要

含钙酸不动杆菌脂肪酶基因的sobria气单胞菌LP004被命名为LP094。wwgs培养基中脂肪酶产酶的研究[j]。Biotechnol。[生物医学工程学报，13:163-166,1997]。通过离子结合将LP094脂肪酶固定在IR120 (Na+)或IRC50 (H+) Amberlite树脂上，在室温(≈25 ~ 30°C)或4°C下保存15 d后，发现其活性保持在90%以上。经过5次重复的脂质水解反应，两种固定化制剂的活性均保持在65%以上。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Production and immobilization of lipase from Aeromonas sobria harboring a heterologous gene

Aeromonas sobria LP004 harboring the Acinetobacter calcoaceticus lipase gene was designated as strain LP094. Lipase production of LP094 in WYGS medium [Lotrakul and Dharmsthiti, World J. Microbiol. Biotechnol., 13 : 163–166, 1997] was scaled up to a 50-l volume. LP094 lipase immobilized by ionic binding to either IR120 (Na⁺) or IRC50 (H⁺) Amberlite resins was found to retain more than 90% activity after 15 d storage at room temperature (≈ 25 to 30°C) or at 4°C. After 5 repeated lipid hydrolysis reactions, the activities of both immobilized preparations remained higher than 65%.

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