海洋绿藻褐藻暗发酵生产乙醇的研究

Yoshiyuki Ueno , Norihide Kurano , Shigetoh Miyachi
{"title":"海洋绿藻褐藻暗发酵生产乙醇的研究","authors":"Yoshiyuki Ueno ,&nbsp;Norihide Kurano ,&nbsp;Shigetoh Miyachi","doi":"10.1016/S0922-338X(98)80031-7","DOIUrl":null,"url":null,"abstract":"<div><p>Dark fermentation in the marine green alga, <em>Chlorococcum littorale</em>, was investigated with emphasis on ethanol production. Under dark anaerobic conditions, 27% of cellular starch was consumed within 24 h at 25°C, the cellular starch decomposition being accelerated at higher temperatures. Ethanol, acetate, hydrogen and carbon dioxide were obtained as fermentation products. The maximum productivity of ethanol was 450 μmol/g-dry wt. at 30°C. The fermentation pathway for cellular starch was proposed from the yields of the end-products and the determined enzyme activities. Ethanol was formed from pyruvate by pyruvate decarboxylase and alcohol dehydrogenase. the change in fermentation pattern that varied with cell concentration in the reaction vials suggested that the hydrogen partial pressure affected the consumption mode of reducing equivalents under dark fermentation. Ethanol productivity was improved by adding methyl viologen, while hydrogen production decreased.</p></div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"86 1","pages":"Pages 38-43"},"PeriodicalIF":0.0000,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80031-7","citationCount":"136","resultStr":"{\"title\":\"Ethanol production by dark fermentation in the marine green alga, Chlorococcum littorale\",\"authors\":\"Yoshiyuki Ueno ,&nbsp;Norihide Kurano ,&nbsp;Shigetoh Miyachi\",\"doi\":\"10.1016/S0922-338X(98)80031-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Dark fermentation in the marine green alga, <em>Chlorococcum littorale</em>, was investigated with emphasis on ethanol production. Under dark anaerobic conditions, 27% of cellular starch was consumed within 24 h at 25°C, the cellular starch decomposition being accelerated at higher temperatures. Ethanol, acetate, hydrogen and carbon dioxide were obtained as fermentation products. The maximum productivity of ethanol was 450 μmol/g-dry wt. at 30°C. The fermentation pathway for cellular starch was proposed from the yields of the end-products and the determined enzyme activities. Ethanol was formed from pyruvate by pyruvate decarboxylase and alcohol dehydrogenase. the change in fermentation pattern that varied with cell concentration in the reaction vials suggested that the hydrogen partial pressure affected the consumption mode of reducing equivalents under dark fermentation. Ethanol productivity was improved by adding methyl viologen, while hydrogen production decreased.</p></div>\",\"PeriodicalId\":15696,\"journal\":{\"name\":\"Journal of Fermentation and Bioengineering\",\"volume\":\"86 1\",\"pages\":\"Pages 38-43\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1998-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80031-7\",\"citationCount\":\"136\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Fermentation and Bioengineering\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0922338X98800317\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Fermentation and Bioengineering","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0922338X98800317","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 136

摘要

研究了海洋绿藻(chlorcoccum littorale)的暗发酵,重点研究了其乙醇的生产。在暗厌氧条件下,在25℃下,27%的细胞淀粉在24 h内被消耗,在更高的温度下,细胞淀粉的分解加速。发酵产物为乙醇、乙酸酯、氢气和二氧化碳。在30℃条件下,乙醇的最大产率为450 μmol/g-dry wt。从最终产物的产率和测定的酶活性两方面提出了细胞淀粉的发酵途径。丙酮酸脱羧酶和乙醇脱氢酶催化丙酮酸合成乙醇。发酵模式随反应瓶中细胞浓度的变化而变化,说明氢分压影响暗发酵条件下还原性当量的消耗模式。添加甲基紫堇提高了乙醇产率,但降低了氢气产率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Ethanol production by dark fermentation in the marine green alga, Chlorococcum littorale

Dark fermentation in the marine green alga, Chlorococcum littorale, was investigated with emphasis on ethanol production. Under dark anaerobic conditions, 27% of cellular starch was consumed within 24 h at 25°C, the cellular starch decomposition being accelerated at higher temperatures. Ethanol, acetate, hydrogen and carbon dioxide were obtained as fermentation products. The maximum productivity of ethanol was 450 μmol/g-dry wt. at 30°C. The fermentation pathway for cellular starch was proposed from the yields of the end-products and the determined enzyme activities. Ethanol was formed from pyruvate by pyruvate decarboxylase and alcohol dehydrogenase. the change in fermentation pattern that varied with cell concentration in the reaction vials suggested that the hydrogen partial pressure affected the consumption mode of reducing equivalents under dark fermentation. Ethanol productivity was improved by adding methyl viologen, while hydrogen production decreased.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Author index Author index Author index Keyword Index Author Index
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1