烟叶半边莲叶提取物作为一种潜在的生物农药防治残叶害虫

B. Deepa, O. Remadevi
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引用次数: 0

摘要

环境问题和全球对有机产品的需求为寻找新的、有效的、安全和经济的农药配方提供了动力。植物为农药的发展提供了巨大的空间。虽然已知有2000多种植物具有杀虫特性,但印楝树是唯一确定的植物杀虫剂。因此,本研究以烟叶有机溶剂提取物为试验对象,对严重的食叶害虫斑蝽幼虫期和卵期的杀虫活性进行了研究。对烟叶有机溶剂提取物的接触毒性和取食毒性进行了评价。25%浓度下,接触毒杀3龄幼虫的死亡率为100%。不同提取物的杀卵活性不同。除乙酸乙酯和水提物外,其余处理均无活性,与对照相当,且在最高浓度(2%)时,孵化抑制率最高,分别为80%和64%。乙酸乙酯提取物和水提取物的lc50值分别为0.55和1.014,杀卵作用不显著(P>0.05)。对4龄幼虫的取食毒性试验表明,烟叶提取物中,甲醇提取物具有极显著的杀虫活性,死亡率为92%,其次是乙醇提取物(90%)和水提取物(86%)。研究表明,烟叶兰作为植物性农药具有巨大的开发潜力。
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Leaf Extracts of Lobelia nicotianaefolia as a Potential Biopesticide Against Defoliator Pests
The environmental concern and global demand for organically produced products provide an impetus to search for new, effective, safe and economical pesticidal formulations. Plants provide enormous scope for development into pesticides. Though more than 2000 plants are known to possess insecticidal properties, neem is the only established botanical pesticide. Hence in this study, we tested the insecticidal activities of the organic solvent extracts from the leaves of L. nicotianaefolia against the larval and egg stages of a serious defoliator pest, Hyblaea puera as the test insect . The organic solvent extracts of the leaf of L. nicotianaefolia were evaluated for their contact and feeding toxicity. The larvicidal action by contact toxicity on 3 rd instar larvae of H. puera showed 100% mortality at 25% concentration. The ovicidal activity varied among the different extracts. All the treatments did not show any activity and were on par with control except ethyl acetate and water extracts and exhibited highest egg hatch inhibition (80%) and (64%) respectively at highest concentration (2%). The LC 50 value for ethyl acetate extract and water extract was 0.55 and 1.014 respectively was not significant (P>0.05) for ovicidal action. The feeding toxicity tests performed on 4 th instar larvae of H. puera showed that among all the extracts of L. nicotianaefolia , methanol extract showed highly significant insecticidal activity with mortality of 92%, followed by ethyl alcohol extract (90%) and water extract (86%). The study reveals that L. nicotianafolia has immense potential to be explored as botanical pesticide.
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