半人马草注射液的hplc - dad - esi - ms分析

V. Nikolić, J. Zvezdanović, S. Konstantinović
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摘要

化学成分估计在茶样品,即冲剂配制从干燥的半人马座赤草。该植物在Stara Planina山(塞尔维亚,42°43'00"N;24°55'04"E),在花期。将植株花顶置于阴暗处干燥至水分含量7.89 (w/w)。采用超高效液相色谱-二极管阵列检测器-电喷雾质谱(UH-PLC-DAD-ESI-MS/MS)方法进行定性分析。本研究的目的是检测和鉴定传统方法制备的冲剂中的植物化学物质,并将其作为生物活性物质和生物抗氧化剂的良好来源。通过对赤藓茶泡茶的分析,发现其主要成分为银环醚萜苷类(獐牙菜苷、龙胆苦苷、龙胆皂苷、獐牙菜苦苷)、山酮类和类黄酮,具有优良的东塞尔维亚茶叶品质。
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UHPLC-DAD-ESI-MS analysis of the 'Centaurium erythraea' infusion
Chemical composition was estimated in a tea sample, i.e. infusion prepared from dried aerial herb of Centaurium erythraea. The plant was harvested in the Stara Planina mountain (Serbia, 42°43'00"N; 24°55'04"E) during the flowering stage. The flowering tops of the plant were dried to the moisture content of 7.89 (w/w) in a dark place. Qualitative analysis was done by Ultrahigh performance liquid chromatography-diode array detector-electrospray mass spectrometry (UH-PLC-DAD-ESI-MS/MS) method. The aim of this study was to detect and identify phytochemicals in the infusion prepared in a traditional way and present it as a good source of biologically active substances and bio-antioxidants. The analysis of C. erythraea infusion primarily indicated the presence of secoiridoid glycosides (sweroside, gentiopicroside, secologanoside, swertiamarin), xanthones and flavo-noids, which promises good quality of tea from Eastern Serbia.
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