Stabilisation of promising breeding rice plants resistant to Pyricularia oryzae

The aim of the research was to create stable breeding material of rice ( Oryza sativa L.) resistant to rice blast ( Pyricularia oryzae ) using the biotechnological method of anther culture in vitro . Anthers from 20 perspective F 2 and F 3 hybrids of carriers of different genes of resistance to rice blast were used. The phytopathological assessment of the rice doubled haploid lines resistance to Pyricularia oryzae was performed in field experiments. Correlation of resistance level to quantitative signs of productivity of the received rice lines was assessed using the statistical analysis. A high sensitivity of all 20 rice genotypes to in vitro conditions was found. The level of callus formation ranged between 3.4% and 82.5% with the average of 23.7%. The level of green plants regeneration from obtained callus varied between 0.16% and 13.3% with the average of 3.3%. The level of albino plants regeneration was 0.6%. On average, approximately 35% of regenerated plants obtained in the culture were able to produce seeds indicating that doubled haploid lines were obtained. Most of them were characterised by a high plant productivity: 21.80–60.42 g compared to 10.31 g for the productivity of standard cultivar ‘Vikont’. A higher productivity was explained by a higher rate of productive bushiness. Eight lines showed a high level of resistance to rice blast (9 points), and 18 lines had an intermediate level of resistance. As a result of the research, perspective lines with a high level of resistance to P. oryzae and increased productivity were obtained. These lines will be used in a further selection programme aimed at obtaining a rice cultivar resistant to P. oryzae .