{"title":"极低频磁场对黑曲霉生长、CMCase、电导率和DNA的影响","authors":"Sahar E. Abo-Neima, M. El-Metwally","doi":"10.21608/ejphysics.2020.29413.1042","DOIUrl":null,"url":null,"abstract":"In the past years the extremely low frequency magnetic field (ELF-MF) of the millimeter range has been actively used in biology, medicine, biotechnology, in this study the impact of electromagnetic radiations in growth, carboxymethycellulase activity (CMCase), cell conductivity and RAPD DNA pattern of A. niger were investigated. Experimentally, when the fungus treated with magnetic field at constant intensity 50Hz, 10 mT at different exposure times for five days (2 h/day) the total biomass dry weight inhibited by 72.2% after the first 2 h. ELF-MF showed also inhibition effect of in CMCase activity of A. niger. The enzyme activity was decreased from 30 IU/ml to 22.5 IU/ml after only 2h of exposure and the treated fungus lost about 50% of its CMCase activity after 10 h.The treated fungus showed also decreased in conductivity of fungal cells from 1.78×10 -1 to 0.51×10 -5 Siemens/meter. The study extended to detect the changes in RAPD patterns of A. niger DNA before and after exposure to the 50 Hz frequency. In conclusion, ELF-MF can be used as a safe method in control the activity of higly resistant fungi as A. niger .","PeriodicalId":22622,"journal":{"name":"The Journal of Applied Sciences Research","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2020-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of extremely low frequency magnetic field in growth, CMCase, electric conductivity and DNA of Aspergillus niger\",\"authors\":\"Sahar E. Abo-Neima, M. El-Metwally\",\"doi\":\"10.21608/ejphysics.2020.29413.1042\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In the past years the extremely low frequency magnetic field (ELF-MF) of the millimeter range has been actively used in biology, medicine, biotechnology, in this study the impact of electromagnetic radiations in growth, carboxymethycellulase activity (CMCase), cell conductivity and RAPD DNA pattern of A. niger were investigated. Experimentally, when the fungus treated with magnetic field at constant intensity 50Hz, 10 mT at different exposure times for five days (2 h/day) the total biomass dry weight inhibited by 72.2% after the first 2 h. ELF-MF showed also inhibition effect of in CMCase activity of A. niger. The enzyme activity was decreased from 30 IU/ml to 22.5 IU/ml after only 2h of exposure and the treated fungus lost about 50% of its CMCase activity after 10 h.The treated fungus showed also decreased in conductivity of fungal cells from 1.78×10 -1 to 0.51×10 -5 Siemens/meter. The study extended to detect the changes in RAPD patterns of A. niger DNA before and after exposure to the 50 Hz frequency. In conclusion, ELF-MF can be used as a safe method in control the activity of higly resistant fungi as A. niger .\",\"PeriodicalId\":22622,\"journal\":{\"name\":\"The Journal of Applied Sciences Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-03-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Journal of Applied Sciences Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21608/ejphysics.2020.29413.1042\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Journal of Applied Sciences Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21608/ejphysics.2020.29413.1042","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Effect of extremely low frequency magnetic field in growth, CMCase, electric conductivity and DNA of Aspergillus niger
In the past years the extremely low frequency magnetic field (ELF-MF) of the millimeter range has been actively used in biology, medicine, biotechnology, in this study the impact of electromagnetic radiations in growth, carboxymethycellulase activity (CMCase), cell conductivity and RAPD DNA pattern of A. niger were investigated. Experimentally, when the fungus treated with magnetic field at constant intensity 50Hz, 10 mT at different exposure times for five days (2 h/day) the total biomass dry weight inhibited by 72.2% after the first 2 h. ELF-MF showed also inhibition effect of in CMCase activity of A. niger. The enzyme activity was decreased from 30 IU/ml to 22.5 IU/ml after only 2h of exposure and the treated fungus lost about 50% of its CMCase activity after 10 h.The treated fungus showed also decreased in conductivity of fungal cells from 1.78×10 -1 to 0.51×10 -5 Siemens/meter. The study extended to detect the changes in RAPD patterns of A. niger DNA before and after exposure to the 50 Hz frequency. In conclusion, ELF-MF can be used as a safe method in control the activity of higly resistant fungi as A. niger .
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