钒吸入对小鼠角膜睫状神经营养因子(CNTF)影响的研究。

IF 1.6 4区 医学 Q3 OPHTHALMOLOGY Cutaneous and Ocular Toxicology Pub Date : 2023-03-01 DOI:10.1080/15569527.2023.2177668
Isis Mendoza-Aldabisis, Nelly López-Valdez, María Eugenia Cervantes-Valencia, Teresa Imelda Fortoul
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引用次数: 1

摘要

目的:空气污染是由掠夺性人类活动和不加选择地燃烧化石燃料造成的公共卫生问题,这些活动将颗粒物(PM)释放到大气中。钒(V)附着在它们上面,吸入后到达血液和不同的器官,如眼睛。另一种接触眼睛的方式是直接接触,角膜是暴露的第一层。睫状神经营养因子(Ciliary neurotrophic factor, CNTF)由角膜神经分泌,其部分功能包括通过激活STAT3来维持自我更新和伤口愈合。本组前期报道表明,吸入V后STAT3被激活,并粘附在PM上。目的:分析吸入V对CNTF表达的影响。方法:CD-1雄性小鼠连续4周和8周吸入V。摘除眼球,对角膜进行CNTF免疫组织化学处理,并进行密度分析。同样的载玻片用于评估组织学改变,并测量角膜前上皮和内皮的厚度。结果:第8周前上皮CNTF表达减少,内皮和角膜厚度增加,前上皮各层紊乱。结论:吸入V扰乱了角膜的结构,改变了CNTF的存在,这可能改变了暴露于PM空气污染后角膜的更新。
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Vanadium inhalation effects on the corneal ciliary neurotrophic factor (CNTF): study in a murine model.

Purpose: Air pollution is a public health problem caused by predatory human activities and the indiscriminate burning of fossil fuels that liberate particulate matter (PM) into the atmosphere. Vanadium (V) adheres to them and reaches the bloodstream and different organs such as the eye when inhaled. Another way to reach the eye is by direct contact, and the cornea is the first layer exposed. Ciliary neurotrophic factor (CNTF) is secreted by the corneal nerves and some of its functions include self-renewal maintenance and wound healing by the activation of STAT3. Previous reports from our group indicate the activation of STAT3 after the inhalation of V, adhered to PM.

Objective: To analyse the effect of V inhalation in the expression of CNTF. Method: CD-1 male mice were exposed for 4 and 8 weeks to V inhalation. The eyes were removed, and the corneas were processed for immunohistochemistry for CNTF and analysed by densitometry. The same slides were used to evaluate histological modifications and to measure the corneas' anterior epithelial and endothelial thickness.

Results: A decrease in CNTF expression in the anterior epithelium in the 8th week, as well as an increase in the endothelial and corneal thickness and disarray of all the layers of the anterior epithelium.

Conclusion: V inhalation disturbs the architecture of the cornea and modifies the presence of CNTF which might modify the renewal of the corneas after exposure to PM air pollution.

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来源期刊
CiteScore
3.30
自引率
6.20%
发文量
40
审稿时长
1 months
期刊介绍: Cutaneous and Ocular Toxicology is an international, peer-reviewed journal that covers all types of harm to cutaneous and ocular systems. Areas of particular interest include pharmaceutical and medical products; consumer, personal care, and household products; and issues in environmental and occupational exposures. In addition to original research papers, reviews and short communications are invited, as well as concise, relevant, and critical reviews of topics of contemporary significance.
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