荧光假单胞菌及刺激性物质对大豆植株生产力及病原菌对种子伤害的影响

M. Solomiychuk, M. Pikovskyi
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引用次数: 2

摘要

大豆是全球农业的战略性豆类,在保障许多国家的粮食安全方面发挥着重要作用。与此同时,生物因素,特别是病原体,阻碍了作物的高质量产量。它们的控制是用各种方法和技术进行的。然而,随着化学农药使用的过度增加,农园的生态状况变得更加复杂。因此,降低农药用量在作物生产技术中具有重要意义。本研究旨在探讨荧光假单胞菌和刺激性物质对大豆植株产量和病原菌对种子伤害的影响。实验在国家植物科学院植物保护研究所乌克兰植物检疫研究站进行。试验田土壤为灰色森林灰化土重壤土。以Xenia大豆品种为研究对象。根据普遍接受的方法进行了实地调查。大豆作物在生长季节的下列时期进行喷施:真三叶期、开花期和成豆期。在处理大豆植株时,使用一种以荧光假单胞菌为基础的制剂,当与具有刺激作用的物质联合使用时,滴度为3 × 109 CFU/cm3,速率为5.0 l/ha。在多年的研究中,降水量(在大豆植物生长季节的某些月份)高于月平均长期标准。没有进行作物收割前的干燥。根据DSTU 4138-2002对不同处理的大豆种子进行了植物病理学检查。研究表明,基于荧光假单胞菌和刺激性物质的药物对大豆植物的生物特征参数及其生产力有积极的影响。喷施植株后,千粒重增加了16 ~ 66 g。大豆种子受枯萎病、赤霉病和青霉病病原菌的侵染率也有所下降——镰刀菌、赤霉菌和扩张青霉。生物复合物组合的免疫保护和刺激作用的研究为开发环境友好型措施以提高大豆产量和减少病原体对种子的伤害提供了前景。
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The influence of Pseudomonas Fluorescens Bacteria and substances of stimulating nature on the productivity of the soybean plant and the damage of seeds by pathogens
Soybeans are a strategic legume for global agriculture and play an important role in ensuring the food security of many countries. At the same time, biotic factors, in particular pathogens, prevent high and high-quality crop yields. Their control is carried out using various methods and techniques. However, with an excessive increase in the use of chemical pesticides, the ecological situation in agrocenoses becomes more complicated. Therefore, it is important to reduce the pesticide load in crop production technologies. The aim of the research is to evaluate the influence of Pseudomonas fluorescens bacteria and substances of stimulating nature on the productivity of soybean plants and seed damage by pathogens. The experiments were performed in the conditions of the Ukrainian Plant Quarantine Research Station of the Institute of Plant Protection of NAAS. The soil of the experimental plot is gray forest podzolic heavy loam. Xenia soybean variety was used for research. Field research was performed according to generally accepted methods. Soybean crops were sprayed during the growing season in the following periods: true trifoliate leaf, flowering and bean formation. For the treatment of soybean plants used a preparation based on bacteria Pseudomonas fluorescens with a titer of 3 x 109 CFU/cm3 with a rate of 5.0 l/ha when used in combination with substances with stimulant action. Over the years of research, the amount of precipitation (in some months of the growing season of soybean plants) was higher than the average monthly long-term norm. Pre-harvest desiccation of crops was not performed. Phytopathological examination of seeds obtained from soybean plants under different treatments was carried out according to DSTU 4138-2002. Studies have shown a positive effect of the drug based on P. fluorescens and substances of a stimulating nature on the biometric parameters of soybean plants and their productivity. In particular, the weight of 1000 seeds in the variants with spraying of plants increased in the range from 16 to 66 g. There was also a decrease in infection of soybean seeds with pathogens of fusarium wilt, Alternaria and penicillosis - fungi Fusarium oxysporum Schlecht, Alternaria spp. and Penicillium expansum Link. The study of immunoprotective and stimulating effects of combinations of biocomplexes is promising for the development of environmentally friendly measures to increase soybean yield and reduce seed damage by pathogens.
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