不同溶剂对铜绿假单胞菌和枯草芽孢杆菌抑菌效果的评价

S. Shikha, Anil Kumar
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摘要

Neolamarckia cadamba在印度是一种常见的树木,以其巨大的绿色叶子和球状的花朵和果实而闻名。此外,树是印度文化和经济上重要的植物,传统上用于制备草药,因为它具有各种药理特性。虽然树皮和树叶大多被利用,而水果在药用方面有些落后。本文研究了新木耳(成熟和未成熟)果实的抗菌特性。采用丙酮、苯、水、DMSO、乙醇和甲醇(mg/ml)等6种不同的溶剂提取物,对cadamba果实对革兰氏阴性菌铜绿假单胞菌和革兰氏阳性菌枯草芽孢杆菌的抑菌性能进行了筛选。结果表明,成熟果实乙醇提取物(14 mm)对枯草芽孢杆菌的抑制区最大。成熟果实的丙酮提取物(5 mm)抑制区最低,未成熟果实的苯和乙醇提取物均无抑制区。在这两种水果中,成熟被发现对两种菌株最有效,而在六种溶剂中,乙醇和甲醇提取物被发现是最有效的。
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Evaluation of Antibacterial Effect against Pseudomonas aeruginosa and Bacillus subtilis Using Different Solvents of Neolamarckia cadamba Fruit Extracts
Neolamarckia cadamba is a commonly found tree in India and is known for its large green leaves and ball shaped flowers and fruits. Also tree is culturally and economically important plant of India and is traditionally used for preparation of herbal medicines because of its various pharmacological properties. Although bark and leaves are mostly utilized whereas fruits are somewhat lagging behind in terms of its medicinal usages. This present study deals with the antibacterial properties of Neolamarckia cadamba (ripe and unripe) fruits. Six different solvent extracts which includes acetone, benzene, water, DMSO, ethanol and methanol (mg/ml) were prepared for the screening of antibacterial properties of Neolamarckia cadamba fruits against gram negative bacteria Pseudomonas aeruginosa and gram positive bacteria Bacillus subtilis. It was observed that zone of inhibition was highest in ethanolic extract (14 mm) of ripe fruit against the Bacillus subtilis. Zone of inhibition was found lowest in acetonic extract (5 mm) of ripe fruits whereas no zone of inhibition was found in benzene and ethanolic extract of unripe fruits. Out of both fruits, ripe was found to be most effective against both strains whereas out of six solvents ethanolic and methanolic extracts were found to be most effective.
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