{"title":"盐杆菌细胞壁糖蛋白:结构、功能和生物合成方面","authors":"Felix Wieland , Johann Lechner, Manfred Sumper","doi":"10.1016/S0721-9571(82)80064-1","DOIUrl":null,"url":null,"abstract":"<div><p>The cell wall glycoprotein of <em>Halobacteria</em> contains sulphate covalenty bound to different types of saccharides. A high molecular weight saccharide (HMW-saccharide), which is N-glycosidically linked to Asn was found to have the following composition: 1 gal: 1 galN: 1 glcN: 2 galUA: 2 SO<sub>4</sub><sup>2-</sup>. This HMW-saccharide is not a heterosaccharide, but exhibits rather a repeating unit structure similar to that of the animal glycosaminoglycans. Incorporation into the cell wall glycoprotein of this HMW-saccharide is specifically inhibited by the antibiotic bacitracin (<em>Wieland</em> et al., 1980). This inhibition results in a remarkable change of the halobacterial shape: the normally rod shaped cells grown in the presence of bacitracin convert to regular spheres (<em>Mescher</em> and <em>Strominger</em>, 1975).</p><p>This HMW-saccharide is synthesized bound to a lipid anchor: only after completion the fully sulphated saccharide is transferred to the core protein.</p></div>","PeriodicalId":101290,"journal":{"name":"Zentralblatt für Bakteriologie Mikrobiologie und Hygiene: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie","volume":"3 1","pages":"Pages 161-170"},"PeriodicalIF":0.0000,"publicationDate":"1982-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0721-9571(82)80064-1","citationCount":"39","resultStr":"{\"title\":\"The Cell Wall Glycoprotein of Halobacteria: Structural, Functional and Biosynthetic Aspects\",\"authors\":\"Felix Wieland , Johann Lechner, Manfred Sumper\",\"doi\":\"10.1016/S0721-9571(82)80064-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The cell wall glycoprotein of <em>Halobacteria</em> contains sulphate covalenty bound to different types of saccharides. A high molecular weight saccharide (HMW-saccharide), which is N-glycosidically linked to Asn was found to have the following composition: 1 gal: 1 galN: 1 glcN: 2 galUA: 2 SO<sub>4</sub><sup>2-</sup>. This HMW-saccharide is not a heterosaccharide, but exhibits rather a repeating unit structure similar to that of the animal glycosaminoglycans. Incorporation into the cell wall glycoprotein of this HMW-saccharide is specifically inhibited by the antibiotic bacitracin (<em>Wieland</em> et al., 1980). This inhibition results in a remarkable change of the halobacterial shape: the normally rod shaped cells grown in the presence of bacitracin convert to regular spheres (<em>Mescher</em> and <em>Strominger</em>, 1975).</p><p>This HMW-saccharide is synthesized bound to a lipid anchor: only after completion the fully sulphated saccharide is transferred to the core protein.</p></div>\",\"PeriodicalId\":101290,\"journal\":{\"name\":\"Zentralblatt für Bakteriologie Mikrobiologie und Hygiene: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie\",\"volume\":\"3 1\",\"pages\":\"Pages 161-170\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1982-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0721-9571(82)80064-1\",\"citationCount\":\"39\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zentralblatt für Bakteriologie Mikrobiologie und Hygiene: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0721957182800641\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zentralblatt für Bakteriologie Mikrobiologie und Hygiene: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0721957182800641","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The Cell Wall Glycoprotein of Halobacteria: Structural, Functional and Biosynthetic Aspects
The cell wall glycoprotein of Halobacteria contains sulphate covalenty bound to different types of saccharides. A high molecular weight saccharide (HMW-saccharide), which is N-glycosidically linked to Asn was found to have the following composition: 1 gal: 1 galN: 1 glcN: 2 galUA: 2 SO42-. This HMW-saccharide is not a heterosaccharide, but exhibits rather a repeating unit structure similar to that of the animal glycosaminoglycans. Incorporation into the cell wall glycoprotein of this HMW-saccharide is specifically inhibited by the antibiotic bacitracin (Wieland et al., 1980). This inhibition results in a remarkable change of the halobacterial shape: the normally rod shaped cells grown in the presence of bacitracin convert to regular spheres (Mescher and Strominger, 1975).
This HMW-saccharide is synthesized bound to a lipid anchor: only after completion the fully sulphated saccharide is transferred to the core protein.
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