尼日利亚奥贡州阿贝奥库塔市出售的家禽饲料中的真菌特征和黄曲霉毒素污染

Kehinde M.T. , Oluwafemi F. , Itoandon E.E. , Orji F.A. , Ajayi O.I.
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引用次数: 18

摘要

由于产生黄曲霉毒素的真菌无处不在,动物饲料中的黄曲霉毒素污染是常见和广泛传播的,特别是在热带地区。对5种动物饲料样品进行了黄曲霉毒素的检测;采用酶联免疫吸附试验(ELISA)。样本取自阿贝奥库塔的五个不同地区。黄曲霉毒素含量以拉芬瓦家禽饲料最高,为93.1 μg/kg;以idia - aba饲料最低,为13.5 μg/kg。真菌数量在4 × 103 ~ 42 × 103 cfu/g之间,拉芬瓦饲料中真菌数量最高,伊迪- aba饲料中真菌数量最低。真菌在马铃薯葡萄糖琼脂(PDA)上生长,分离鉴定出黄曲霉、米曲霉、米根霉和青霉,以黄曲霉为主。采用方差分析(ANOVA)进行比较统计分析,95%置信区间均值差异显著。综上所述,所有样品中均存在黄曲霉毒素,即使浓度很低,也会对人类和动物健康造成严重影响。玉米是所有受污染饲料的主要成分。
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Fungal Profile and Aflatoxin Contamination in Poultry Feeds Sold in Abeokuta, Ogun State, Nigeria

Aflatoxin contamination of animal feeds is common and widely spread, especially in the tropics, due to the ubiquity of the producing fungi. The detection of aflatoxin in five samples of animal feed was carried out; using enzyme linked immunosorbent assay (ELISA). Samples were taken from five different areas in Abeokuta. The aflatoxin level was observed to be the highest in the poultry feed from Lafenwa with the value 93.1 μg/kg; and lowest in the feed from Idi-Aba with the value 13.5 μg/kg. Fungal counts are between 4 × 103 and 42 × 103 cfu/g, with highest count occurring in the feed from Lafenwa and lowest in Idi-Aba. The fungal growth was on potato dextrose agar (PDA), and Aspergillus flavus, A. oryzae, Rhizopus oryzae and Penicillum notatum were isolated and identified, with Aspergillus flavus predominating. Comparison statistical analysis using ANOVA showed a significant mean difference 95% confidence interval. In conclusion, aflatoxin was present in all the samples, which even at low concentration is of great concern to human and animal health. Maize was the main ingredient in all the contaminated feed.

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