Mark R Viant, Eric S Rosenblum, Ronald S Tjeerdema
{"title":"高效液相色谱法测定鲍鱼组织中磷酸甘氨酸的优化方法","authors":"Mark R Viant, Eric S Rosenblum, Ronald S Tjeerdema","doi":"10.1016/S0378-4347(01)00428-5","DOIUrl":null,"url":null,"abstract":"<div><p>A rapid high-performance liquid chromatography method for the determination of phosphoarginine (PArg) in invertebrate tissue has been redeveloped and validated. The method employs a reversed-phase amino column and a KH<sub>2</sub>PO<sub>4</sub>–acetonitrile mobile phase. PArg peak identity was confirmed by comparison with a known standard and via enzymatic conversion. Additionally linear calibration data, low intra-assay variability (<4%), and a detection limit of 5 pmol were determined. The method was demonstrated using PArg extracted from red abalone (<em>Haliotis rufescens</em>) adductor muscle. Validation of the extraction procedure was also completed, including the measurement of a 100.2±0.9% extraction efficiency.</p></div>","PeriodicalId":15463,"journal":{"name":"Journal of Chromatography B: Biomedical Sciences and Applications","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2001-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0378-4347(01)00428-5","citationCount":"41","resultStr":"{\"title\":\"Optimized method for the determination of phosphoarginine in abalone tissue by high-performance liquid chromatography\",\"authors\":\"Mark R Viant, Eric S Rosenblum, Ronald S Tjeerdema\",\"doi\":\"10.1016/S0378-4347(01)00428-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A rapid high-performance liquid chromatography method for the determination of phosphoarginine (PArg) in invertebrate tissue has been redeveloped and validated. The method employs a reversed-phase amino column and a KH<sub>2</sub>PO<sub>4</sub>–acetonitrile mobile phase. PArg peak identity was confirmed by comparison with a known standard and via enzymatic conversion. Additionally linear calibration data, low intra-assay variability (<4%), and a detection limit of 5 pmol were determined. The method was demonstrated using PArg extracted from red abalone (<em>Haliotis rufescens</em>) adductor muscle. Validation of the extraction procedure was also completed, including the measurement of a 100.2±0.9% extraction efficiency.</p></div>\",\"PeriodicalId\":15463,\"journal\":{\"name\":\"Journal of Chromatography B: Biomedical Sciences and Applications\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2001-12-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0378-4347(01)00428-5\",\"citationCount\":\"41\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Chromatography B: Biomedical Sciences and Applications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0378434701004285\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Chromatography B: Biomedical Sciences and Applications","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0378434701004285","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Optimized method for the determination of phosphoarginine in abalone tissue by high-performance liquid chromatography
A rapid high-performance liquid chromatography method for the determination of phosphoarginine (PArg) in invertebrate tissue has been redeveloped and validated. The method employs a reversed-phase amino column and a KH2PO4–acetonitrile mobile phase. PArg peak identity was confirmed by comparison with a known standard and via enzymatic conversion. Additionally linear calibration data, low intra-assay variability (<4%), and a detection limit of 5 pmol were determined. The method was demonstrated using PArg extracted from red abalone (Haliotis rufescens) adductor muscle. Validation of the extraction procedure was also completed, including the measurement of a 100.2±0.9% extraction efficiency.
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