提高国内反刍动物细菌和真菌流产剂回收率的方法。

IF 1.5 3区 农林科学 Q2 VETERINARY SCIENCES Onderstepoort Journal of Veterinary Research Pub Date : 2023-01-11 DOI:10.4102/ojvr.v90i1.2010
Annelize Jonker, Peter N Thompson, Anita L Michel
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引用次数: 1

摘要

家养反刍动物的流产给农民造成了巨大的经济损失。确定流产的原因对控制措施很重要,但这可能具有挑战性。由于许多限制因素(自溶、缺乏病史、样本范围)使调查过程复杂化,应在每个病例中使用细菌学实验室中所有可用的诊断方法。本研究的目的是确定是否可以通过结合现有的好氧培养和布鲁氏菌选择性方法,以及文献报道流产调查中普遍推荐的方法,来增加国内反刍动物流产病例中诊断意义重大的分离株的回收率。这些方法是检查湿制剂和印迹涂片染色的改进Ziehl-Neelsen法,厌氧,嗜微气,钩端螺旋体,支原体和真菌培养。采用新方法对135例牛(88例)、绵羊(25例)和山羊(22例)的胎盘和流产胎儿样本进行分析。在46例病例中,细菌被鉴定为病原,1例为真菌。与前10年相比,布鲁氏菌的分离率增加了7.4%,空肠弯曲杆菌(n = 2)和根霉(n = 1)的分离率也增加了,沙门氏菌(5.9%)和化脓性Trueperella(4.4%)的分离率也增加了。总之,该方法可以有效地消除细菌学实验室的检测选择偏差。通过广泛结合传统培养方法对流产产物进行深入研究的重要性,通过增加流产布鲁氏菌和空肠梭菌的分离来强调。产生最具临床相关性的分离菌株的方法组合是好氧、嗜微气、布鲁氏菌和真菌培养。
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Approaches to increase recovery of bacterial and fungal abortion agents in domestic ruminants.

Abortions in domestic ruminants cause significant economic losses to farmers. Determining the cause of an abortion is important for control efforts, but it can be challenging. All available diagnostic methods in the bacteriology laboratory should be employed in every case due to the many limiting factors (autolysis, lack of history, range of samples) that complicate the investigation process. The purpose of this study was to determine whether the recovery of diagnostically significant isolates from domestic ruminant abortion cases could be increased through the use of a combination of the existing aerobic culture and Brucella selective method with methods that are commonly recommended in the literature reporting abortion investigations. These methods are examination of wet preparations and impression smears stained by the modified Ziehl-Neelsen method, anaerobic, microaerophilic, Leptospira, Mycoplasma and fungal culture. Samples of placenta and aborted foetuses from 135 routine clinical abortion cases of cattle (n = 88), sheep (n = 25) and goats (n = 22) were analysed by the new combination of methods. In 46 cases, bacteria were identified as aetiological agents and in one case a fungus. Isolation of Brucella species increased to 7.4% over two years compared with the previous 10 years (7.3%), as well as Campylobacter jejuni (n = 2) and Rhizopus species (n = 1). Salmonella species (5.9%) and Trueperella pyogenes (4.4%) were also isolated more often. In conclusion, the approach was effective in removing test selection bias in the bacteriology laboratory. The importance of performing an in-depth study on the products of abortion by means of an extensive, combination of conventional culture methods was emphasised by increased isolation of Brucella abortus and isolation of C. jejuni. The combination of methods that yielded the most clinically relevant isolates was aerobic, microaerophilic, Brucella and fungal cultures.

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来源期刊
CiteScore
4.30
自引率
0.00%
发文量
13
审稿时长
16 weeks
期刊介绍: The Onderstepoort Journal of Veterinary Research, is the official publication of the Onderstepoort Veterinary Institute. While it considers submissions from any geographic region, its focus is on Africa and the infectious and parasitic diseases and disease vectors that affect livestock and wildlife on the continent.
期刊最新文献
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