微滴数字PCR与evgreen分析:结构变异的确证分析。

Current Protocols in Human Genetics Pub Date : 2018-04-01 DOI:10.1002/cphg.58

Angela C Tai, Michael Parfenov, Joshua M Gorham

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引用次数: 5

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Droplet Digital PCR with EvaGreen Assay: Confirmational Analysis of Structural Variants.

DNA structural variants can be analyzed by droplet digital PCR (ddPCR), a water-oil microfluidics and fluorescence technology to quantify target nucleic acids with extreme precision and sensitivity. Traditional ddPCR uses expensive fluorescent oligonucleotide probes that require extensive optimization. Here we describe a variation of ddPCR using a DNA-binding dye (EvaGreen), whose properties allow target products to be effectively quantified at a significantly lower cost. © 2018 by John Wiley & Sons, Inc.

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Current Protocols in Human Genetics Biochemistry, Genetics and Molecular Biology-Genetics

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期刊介绍： Current Protocols in Human Genetics is the resource for designing and running successful research projects in all branches of human genetics.