近红外光处理后mptp处理小鼠多巴胺能无毛细胞的存活。

ISRN Neurology Pub Date : 2012-01-01 DOI:10.5402/2012/850150
Cassandra Peoples, Victoria E Shaw, Jonathan Stone, Glen Jeffery, Gary E Baker, John Mitrofanis
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引用次数: 29

摘要

在急性和慢性1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)帕金森病小鼠模型中,我们研究了近红外光(NIr)治疗(光生物调节)是否能保存视网膜的多巴胺能无分泌细胞。对于急性模型,BALB/c小鼠在30小时内注射MPTP (100 mg/kg)或生理盐水,然后进行6天的生存期。对于慢性模型,小鼠在五周内注射MPTP (200 mg/kg)或生理盐水,然后进行三周的生存期。NIr治疗可在MPTP损伤的同时(同时系列)或在MPTP损伤后(治疗后系列)应用。每个系列中有四组:Saline, salt - nir, MPTP和MPTP- nir。对视网膜进行酪氨酸羟化酶(TH)免疫化学处理,分析细胞数量。在MPTP组中,与生理盐水对照组相比,TH(+)细胞数量显著减少;这种减少在急性(~50%)比慢性(~30%)病例更大。在MPTP- nir组中,TH(+)细胞明显多于MPTP组(~30%)。总之,我们发现NIr治疗能够保护(同时系列)和拯救(治疗后系列)视网膜TH(+)细胞免受帕金森损伤。
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Survival of Dopaminergic Amacrine Cells after Near-Infrared Light Treatment in MPTP-Treated Mice.

We examined whether near-infrared light (NIr) treatment (photobiomodulation) saves dopaminergic amacrine cells of the retina in an acute and a chronic 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse model of Parkinson disease. For the acute model, BALB/c mice had MPTP (100 mg/kg) or saline injections over 30 hours, followed by a six-day-survival period. For the chronic model, mice had MPTP (200 mg/kg) or saline injections over five weeks, followed by a three-week-survival period. NIr treatment was applied either at the same time (simultaneous series) or well after (posttreatment series) the MPTP insult. There were four groups within each series: Saline, Saline-NIr, MPTP, and MPTP-NIr. Retinae were processed for tyrosine hydroxylase (TH) immunochemistry, and cell number was analysed. In the MPTP groups, there was a significant reduction in TH(+) cell number compared to the saline controls; this reduction was greater in the acute (~50%) compared to the chronic (~30%) cases. In the MPTP-NIr groups, there were significantly more TH(+) cells than in the MPTP groups of both series (~30%). In summary, we showed that NIr treatment was able to both protect (simultaneous series) and rescue (posttreatment series) TH(+) cells of the retina from parkinsonian insult.

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