醋酸和胃蛋白酶溶解鹦嘴鱼鱼鳞中I型胶原蛋白的提取及特性研究。

IF 3 Q3 MATERIALS SCIENCE, BIOMATERIALS International Journal of Biomaterials Pub Date : 2023-01-01 DOI:10.1155/2023/7312447
Abdul Aziz Jaziri, Rossita Shapawi, Ruzaidi Azli Mohd Mokhtar, Wan Norhana Md Noordin, Nurul Huda
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引用次数: 1

摘要

海鱼加工产生的废物是有价值产品的重要来源。鱼类胶原蛋白因其优异的性能被认为是一种可替代的生物材料,被广泛用于工业用途。因此,本研究旨在从鹦嘴鱼(Scarus sordidus forssk, 1775)鱼鳞废弃物中提取酸和胃蛋白酶可溶性胶原。酸溶性胶原蛋白(ASC-PFS)和胃蛋白酶可溶性胶原蛋白(PSC-PFS)的产率分别为1.17 g/100 g和1.00 g/100 g (p > 0.05)。经十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)证实,两种胶原蛋白均存在α1和α1两个α链亚基,均为I型。在傅里叶红外(FTIR)测试下,ASC-PFS和PSC-PFS的I型胶原保持了三螺旋结构。此外,对不同鱼种胶原的紫外可见光谱和x射线衍射(XRD)的研究表明,不同鱼种胶原具有相似性,提取的所有胶原的热稳定性(T max)评价范围在36.22 ~ 37.78°C之间,其值与前人对鱼鳞胶原的研究相当。不同pH和氯化钠(NaCl)处理对其溶解度的影响表明,ASC-PFS和PSC-PFS在酸性条件下(pH
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Extraction and Characterization of Type I Collagen from Parrotfish (Scarus sordidus Forsskål, 1775) Scale solubilized with the Aid of Acetic Acid and Pepsin.

Waste from marine fish processing is an important source of valuable products. Fish collagen is considered a alternative biomaterial due to its excellent properties, and it is widely used for industrial purposes. Thus, this present study aimed to characterize acid and pepsin-soluble collagens from the waste of parrotfish (Scarus sordidus Forsskål, 1775) scales. The yields (p > 0.05) of acid-soluble collagen (ASC-PFS) and pepsin-soluble collagen (PSC-PFS) were 1.17 g/100 g and 1.00 g/100 g, respectively. Both collagen samples were categorized as type I owing to the presence of two alpha chain subunits (α1 and α1) after being confirmed by a sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Under the fourier transform infrared (FTIR) test, the triple helical structure of type I collagens from the ASC-PFS and PSC-PFS was maintained. Moreover, the study of UV visible spectra and X-ray diffraction (XRD) showed the similarity of collagens derived from different fish species, and the thermostability (T max) evaluation of all extracted collagens was in the range of 36.22-37.78°C, and their values were comparable to previous research on the fish scale collagens. The effect of various pH and sodium chloride (NaCl) treatments on solubility exhibited that the ASC-PFS and PSC-PFS were highly soluble in an acidic condition (pH < 5.0) and low concentration of sodium chloride (<30 g/L). Taken together, collagens extracted from parrotfish scale waste can be an alternative source for industries.

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来源期刊
International Journal of Biomaterials
International Journal of Biomaterials MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
4.30
自引率
3.20%
发文量
50
审稿时长
21 weeks
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