小鼠发情周期中雌性生殖系统中IRS2的表达。

IF 1.6 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnic & Histochemistry Pub Date : 2023-04-01 DOI:10.1080/10520295.2022.2153167

Zhongli Wang, Benyi Yi, Lijun Gan, Xiuli Li, Xiuxiu Liu, Qizhuang Lv, Lei Yang

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引用次数: 0

摘要

胰岛素受体底物2 (IRS2)参与生殖;然而，IRS2在雌性小鼠生殖系统中的位置和表达尚不清楚。采用实时定量聚合酶链反应(RT-PCR)、免疫印迹(western blot)和免疫组化(immunohistochemical)染色技术研究了IRS2在雌性小鼠发情周期卵巢、输卵管和子宫中的表达情况。我们发现IRS2在小鼠的所有生殖器官中均有表达，且表达水平随发情期的变化而变化。IRS2在生殖器官的表达在发情期最高。

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Expression of IRS2 in the female reproductive system during the estrous cycle in mice.

Insulin receptor substrate 2 (IRS2) participates in reproduction; however, the location and expression of IRS2 in the reproductive system of female mice is not clear. We used real-time quantitative polymerase chain reaction (RT-PCR), western blot and immunohistochemical staining to investigate the expression of IRS2 in the ovary, oviduct and uterus of female mice during the estrous cycle. We found that IRS2 was expressed in all reproductive organs of mouse and that the expression level changed with the estrous phases. The expression of IRS2 in reproductive organs was greatest during estrus.

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来源期刊

Biotechnic & Histochemistry 生物-生物工程与应用微生物

CiteScore

3.40

自引率

6.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Biotechnic & Histochemistry (formerly Stain technology) is the official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926. Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well. Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.