{"title":"[耐力运动对小鼠骨骼肌葡萄糖摄取的影响及白细胞介素的调节机制]。","authors":"Zhe Wang, Qian Wang, Hua-Duo Wu, Yong-Qiang Hu, Zi-Fu Peng, Xiang-Ying Guo, Ning Jiang","doi":"10.12047/j.cjap.6360.2022.126","DOIUrl":null,"url":null,"abstract":"目的: 探讨耐力运动对小鼠骨骼肌葡萄糖摄取能力的影响及白细胞介素(IL-15)的作用机制。方法: 将32只C57BL/6J小鼠随机分为安静对照组(Control, C)、耐力训练组(Exercise, E)、PBS注射组(PBS injected, PI)、IL-15注射组(IL-15 injected, II)。每组8只,C组小鼠正常饲养8周,E组小鼠进行8周中等强度耐力训练,II组小鼠一次性腹腔注射300 μl IL-15复合剂溶液(1.5 μg IL-15和7 μg IL-15Rα),PI组一次性腹腔注射与II组等量的PBS。其中C组和E组在饲养第7周时,PI组和II组在一次性注射后,各组小鼠禁食12 h,随后进行葡萄糖耐量试验(IPGTT)检测并记录血糖水平。采用Western blot检测小鼠骨骼肌GLUT4、IL-15、AKT、pAKT、AMPK、pAMPK、AS160、pAS160的蛋白表达水平。结果: 与C组相比,E组小鼠骨骼肌IL-15、GLUT4、pAMPK、pAKT、pAS160蛋白表达明显升高(P＜0.05)。与PI组相比,II组小鼠骨骼肌IL-15、GLUT4、pAMPK、pAS160蛋白表达明显升高(P＜0.05)。结论: 8周耐力运动和腹腔注射IL-15复合剂均可提升小鼠骨骼肌葡萄糖的摄取能力,但单独增加IL-15可能是通过激活AMPK/AS160/GLUT4通路提升对于葡萄糖的转运能力,这一过程不依赖AKT/AS160/GLUT4通路的激活。.","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Effects of endurance exercise on glucose uptake of skeletal muscle in mice and regulation mechanism of interleukin 15].\",\"authors\":\"Zhe Wang, Qian Wang, Hua-Duo Wu, Yong-Qiang Hu, Zi-Fu Peng, Xiang-Ying Guo, Ning Jiang\",\"doi\":\"10.12047/j.cjap.6360.2022.126\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"目的: 探讨耐力运动对小鼠骨骼肌葡萄糖摄取能力的影响及白细胞介素(IL-15)的作用机制。方法: 将32只C57BL/6J小鼠随机分为安静对照组(Control, C)、耐力训练组(Exercise, E)、PBS注射组(PBS injected, PI)、IL-15注射组(IL-15 injected, II)。每组8只,C组小鼠正常饲养8周,E组小鼠进行8周中等强度耐力训练,II组小鼠一次性腹腔注射300 μl IL-15复合剂溶液(1.5 μg IL-15和7 μg IL-15Rα),PI组一次性腹腔注射与II组等量的PBS。其中C组和E组在饲养第7周时,PI组和II组在一次性注射后,各组小鼠禁食12 h,随后进行葡萄糖耐量试验(IPGTT)检测并记录血糖水平。采用Western blot检测小鼠骨骼肌GLUT4、IL-15、AKT、pAKT、AMPK、pAMPK、AS160、pAS160的蛋白表达水平。结果: 与C组相比,E组小鼠骨骼肌IL-15、GLUT4、pAMPK、pAKT、pAS160蛋白表达明显升高(P＜0.05)。与PI组相比,II组小鼠骨骼肌IL-15、GLUT4、pAMPK、pAS160蛋白表达明显升高(P＜0.05)。结论: 8周耐力运动和腹腔注射IL-15复合剂均可提升小鼠骨骼肌葡萄糖的摄取能力,但单独增加IL-15可能是通过激活AMPK/AS160/GLUT4通路提升对于葡萄糖的转运能力,这一过程不依赖AKT/AS160/GLUT4通路的激活。.\",\"PeriodicalId\":23985,\"journal\":{\"name\":\"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.12047/j.cjap.6360.2022.126\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.12047/j.cjap.6360.2022.126","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0
[Effects of endurance exercise on glucose uptake of skeletal muscle in mice and regulation mechanism of interleukin 15].
目的: 探讨耐力运动对小鼠骨骼肌葡萄糖摄取能力的影响及白细胞介素(IL-15)的作用机制。方法: 将32只C57BL/6J小鼠随机分为安静对照组(Control, C)、耐力训练组(Exercise, E)、PBS注射组(PBS injected, PI)、IL-15注射组(IL-15 injected, II)。每组8只,C组小鼠正常饲养8周,E组小鼠进行8周中等强度耐力训练,II组小鼠一次性腹腔注射300 μl IL-15复合剂溶液(1.5 μg IL-15和7 μg IL-15Rα),PI组一次性腹腔注射与II组等量的PBS。其中C组和E组在饲养第7周时,PI组和II组在一次性注射后,各组小鼠禁食12 h,随后进行葡萄糖耐量试验(IPGTT)检测并记录血糖水平。采用Western blot检测小鼠骨骼肌GLUT4、IL-15、AKT、pAKT、AMPK、pAMPK、AS160、pAS160的蛋白表达水平。结果: 与C组相比,E组小鼠骨骼肌IL-15、GLUT4、pAMPK、pAKT、pAS160蛋白表达明显升高(P＜0.05)。与PI组相比,II组小鼠骨骼肌IL-15、GLUT4、pAMPK、pAS160蛋白表达明显升高(P＜0.05)。结论: 8周耐力运动和腹腔注射IL-15复合剂均可提升小鼠骨骼肌葡萄糖的摄取能力,但单独增加IL-15可能是通过激活AMPK/AS160/GLUT4通路提升对于葡萄糖的转运能力,这一过程不依赖AKT/AS160/GLUT4通路的激活。.
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