用于人类疾病诊断、预后和治疗分析的组织切片技术比较。

IF 3.5 4区 医学 Q3 CELL BIOLOGY Pathobiology Pub Date : 2023-01-01 Epub Date: 2022-08-11 DOI:10.1159/000525979
Elise M Walsh, Marc K Halushka
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引用次数: 0

摘要

组织病理学历来是诊断和治疗人类疾病的关键技术。如今,从特定细胞而不是大块组织中提取基因组学、转录组学和蛋白质组学已成为了解潜在疾病机制和提供有用诊断信息的关键。从易于获取的福尔马林固定石蜡包埋组织中提取所需的分析物,即核酸或蛋白质,可用于临床相关活动,如生物标记突变测序或淀粉样蛋白分型。基因图谱分析已成为非小细胞肺癌和前列腺癌等各种癌症的常规检测方法。目前已开发出的五种主要组织解剖技术包括:批量刮取、人工大切片、人工显微切片、激光捕获显微切片和表达显微切片。在这篇综述中,我们将讨论组织切片在临床实践和研究中的重要性、基本方法、应用以及每种方法的优缺点。
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A Comparison of Tissue Dissection Techniques for Diagnostic, Prognostic, and Theragnostic Analysis of Human Disease.

Histopathology has historically been the critical technique for the diagnosis and treatment of human disease. Today, genomics, transcriptomics, and proteomics from specific cells, rather than bulk tissue, have become key to understanding underlying disease mechanisms and rendering useful diagnostic information. Extraction of desired analytes, i.e., nucleic acids or proteins, from easily accessible formalin-fixed paraffin-embedded tissues allows for clinically relevant activities, such as sequencing biomarker mutations or typing amyloidogenic proteins. Genetic profiling has become routine for cancers as varied as non-small cell lung cancer and prostatic carcinoma. The five main tissue dissection techniques that have been developed thus far include: bulk scraping, manual macrodissection, manual microdissection, laser-capture microdissection, and expression microdissection. In this review, we discuss the importance of tissue dissection in clinical practice and research, the basic methods, applications, as well as some advantages and disadvantages for each modality.

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来源期刊
Pathobiology
Pathobiology 医学-病理学
CiteScore
8.50
自引率
0.00%
发文量
47
审稿时长
>12 weeks
期刊介绍: ''Pathobiology'' offers a valuable platform for the publication of high-quality original research into the mechanisms underlying human disease. Aiming to serve as a bridge between basic biomedical research and clinical medicine, the journal welcomes articles from scientific areas such as pathology, oncology, anatomy, virology, internal medicine, surgery, cell and molecular biology, and immunology. Published bimonthly, ''Pathobiology'' features original research papers and reviews on translational research. The journal offers the possibility to publish proceedings of meetings dedicated to one particular topic.
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