非复发性和复发性肿瘤光动力疗法前基底细胞癌中β-Catenin、E-Cadherin和α-平滑肌肌动蛋白的表达:探索光动力疗法结果的预测能力

IF 1.9 4区 生物学 Q4 CELL BIOLOGY Journal of Histochemistry & Cytochemistry Pub Date : 2023-03-01 Epub Date: 2023-03-24 DOI:10.1369/00221554231161396
Erik Mørk, Patricia Mjønes, Olav A Foss, Ingeborg M Bachmann, Eidi Christensen
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引用次数: 0

摘要

光动力疗法(PDT)是治疗低危基底细胞癌(BCC)的一种有效且有益于美容的方法。为了优化光动力疗法的反应,正确选择肿瘤非常重要。除了临床和组织学检查外,我们还试图找到能识别此类肿瘤的标记物。研究表明,β-catenin、E-cadherin 和 α-平滑肌肌动蛋白(SMA)的表达可显示 BCC 的侵袭性/侵袭性。我们希望在一项探索性研究中使用这些标记物,研究它们在非复发性 BCC 与复发性 BCC 中的表达是否有所不同,以评估它们预测 PDT 结果的能力。研究人员用β-catenin、E-cadherin和α-SMA抗体对52个BCC进行了染色,并用免疫反应评分(IRS)、亚细胞定位和基质蛋白表达进行了评估。结果显示,复发性 BCC 与非复发性 BCC 相比,E-cadherin 的 IRS 有显著差异,接收者操作特征曲线下面积为 0.71(95% 置信区间:0.56-0.86,P=0.025)。复发性 BCC 的基质 β-catenin 表达明显增加。一些复发的 BCC 在肿瘤深层浸润边缘有高表达。总之,E-cadherin、基质和深部边缘β-catenin的表达在PDT后复发的BCC中最为突出,这表明它们有可能预测PDT的结果。这些结果是否具有临床价值还需要进一步研究。
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Expression of β-Catenin, E-Cadherin, and α-Smooth Muscle Actin in Basal Cell Carcinoma Before Photodynamic Therapy in Non-recurrent and Recurrent Tumors: Exploring the Ability of Predicting Photodynamic Therapy Outcome.

Photodynamic therapy (PDT) is an effective and cosmetically beneficial treatment of low-risk basal cell carcinomas (BCCs). To optimize PDT response, it is important to correctly select tumors. We sought to find markers that could identify such tumors beyond contributions from clinical and histological examination. Studies have shown that β-catenin, E-cadherin, and α-smooth muscle actin (SMA) expression can indicate BCC aggressiveness/BCC invasiveness. We wanted to use these markers in an explorative study to investigate whether they were differently expressed among non-recurring compared with recurring BCCs, to evaluate their ability of predicting PDT outcome. Fifty-two BCCs were stained with antibodies against β-catenin, E-cadherin, and α-SMA, and evaluated using immunoreactive score (IRS), subcellular localization, and stromal protein expression. Results showed that IRS of E-cadherin was significantly different among recurring compared with non-recurring BCCs and with area under a receiver operating characteristic curve of 0.71 (95% confidence interval: 0.56-0.86, p=0.025). Stromal β-catenin expression significantly increased among recurring BCCs. Some recurring BCCs had intense expression in the deep invading tumor edge. In conclusion, E-cadherin, and stromal and deep edge β-catenin expression were most prominent in BCCs that recurred post-PDT, suggesting they could potentially predict PDT outcome. Further studies are needed to investigate whether these results are of clinical value.

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来源期刊
CiteScore
6.00
自引率
0.00%
发文量
32
审稿时长
1 months
期刊介绍: Journal of Histochemistry & Cytochemistry (JHC) has been a pre-eminent cell biology journal for over 50 years. Published monthly, JHC offers primary research articles, timely reviews, editorials, and perspectives on the structure and function of cells, tissues, and organs, as well as mechanisms of development, differentiation, and disease. JHC also publishes new developments in microscopy and imaging, especially where imaging techniques complement current genetic, molecular and biochemical investigations of cell and tissue function. JHC offers generous space for articles and recognizing the value of images that reveal molecular, cellular and tissue organization, offers free color to all authors.
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