结合菌在草地上定植的定量研究

Q1 Agricultural and Biological Sciences Current protocols in plant biology Pub Date : 2017-06-21 DOI:10.1002/cppb.20047

Eduardo Balsanelli, Fábio de Oliveira Pedrosa, Emanuel Maltempi de Souza

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Quantification of Grass Colonization by Associative Bacteria

There is a growing interest in the use of plant growth–promoting rhizobacteria to improve crop productivity as a partial substitute for nitrogen fertilizer. Bacteria-colonizing plants may be epiphytic or endophytic. This article describes reproducible protocols to quantify the level of colonization in each plant compartment. The protocols were developed using several cereal crops such as maize, rice, sorghum, and wheat. © 2017 by John Wiley & Sons, Inc.

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Current protocols in plant biology Agricultural and Biological Sciences-Plant Science

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. Current Protocols in Plant Biology provides reproducible step-by-step instructions for protocols relevant to plant research. Furthermore, Current Protocols content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols in Plant Biology to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Plant Biology is the comprehensive source for protocols in the multidisciplinary field of plant biology, providing an extensive range of protocols from basic to cutting edge. Coverage includes: Extraction and analysis of DNA, RNA, proteins Chromosome analysis Transcriptional analysis Protein expression Metabolites Plant enzymology Epigenetics Plant genetic transformation Mutagenesis Arabidopsis, Maize, Poplar, Rice, and Soybean, and more.