揭示线粒体外膜通透性和膜电位:最新的方法和生物传感器

IF 6.2 2区 生物学 Q1 CELL BIOLOGY Seminars in cell & developmental biology Pub Date : 2023-07-10 DOI:10.1016/j.semcdb.2023.07.003
Nikolay Popgeorgiev , Clara Gil , Kevin Berthenet , Giulia Bertolin , Gabriel Ichim
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引用次数: 4

摘要

膜结构完整性对于优化线粒体功能至关重要。这些细胞器产生所有生命过程所需的能量,只要它们的内外膜完好无损。这可以防止线粒体凋亡因子释放到细胞质中,并确保完整的线粒体膜电位(ΔΨm)来维持ATP的产生。细胞凋亡通常由外线粒体膜透性(MOMP)触发,并与ΔΨ m的丢失紧密结合。由于这两个过程对线粒体功能和细胞死亡都至关重要,研究人员设计了各种技术来评估它们。在这里,我们讨论了目前用于检测MOMP和测量ΔΨ m的方法和生物传感器,重点讨论了它们的优点和局限性,并讨论了需要哪些新的成像工具来提高我们对线粒体功能的了解。
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Shedding light on mitochondrial outer-membrane permeabilization and membrane potential: State of the art methods and biosensors

Membrane structural integrity is essential for optimal mitochondrial function. These organelles produce the energy needed for all vital processes, provided their outer and inner membranes are intact. This prevents the release of mitochondrial apoptogenic factors into the cytosol and ensures intact mitochondrial membrane potential (ΔΨm) to sustain ATP production. Cell death by apoptosis is generally triggered by outer mitochondrial membrane permeabilization (MOMP), tightly coupled with loss of ΔΨ m. As these two processes are essential for both mitochondrial function and cell death, researchers have devised various techniques to assess them. Here, we discuss current methods and biosensors available for detecting MOMP and measuring ΔΨ m, focusing on their advantages and limitations and discuss what new imaging tools are needed to improve our knowledge of mitochondrial function.

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来源期刊
CiteScore
15.10
自引率
1.40%
发文量
310
审稿时长
9.1 weeks
期刊介绍: Seminars in Cell and Developmental Biology is a review journal dedicated to keeping scientists informed of developments in the field of molecular cell and developmental biology, on a topic by topic basis. Each issue is thematic in approach, devoted to an important topic of interest to cell and developmental biologists, focusing on the latest advances and their specific implications. The aim of each issue is to provide a coordinated, readable, and lively review of a selected area, published rapidly to ensure currency.
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