银纳米颗粒掺入用于牙科应用的聚甲基丙烯酸甲酯的抗生物膜特性。

IF 1 4区 医学 Q4 ENGINEERING, BIOMEDICAL Bio-medical materials and engineering Pub Date : 2023-01-01 DOI:10.3233/BME-222513
Marine Ortiz-Magdaleno, Luis Sánchez-Vargas, Delia Gardea-Contreras, Verónica Campos-Ibarra, Amaury Pozos-Guillén, Raúl Márquez-Preciado
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引用次数: 0

摘要

背景:用于牙科和生物医学应用的丙烯酸树脂不具有抗菌性能,它们的表面容易受到微生物定植的影响。目的:研究沉积在聚甲基丙烯酸甲酯(PMMA)表面的银纳米粒子(AgNPs)对金黄色葡萄球菌生物膜的抗菌性能。方法:采用原位聚合法制备AgNPs。采用原子吸收分光光度法(AAS)测定掺入银(Ag+)在24 h、48 h、7 d和30 d的溶解度。采用金黄色葡萄球菌细胞悬浮液,在CDC生物膜生物反应器系统中组装30份含AgNPs和不含NP的PMMA标本(对照组)。在6、12、24、48和72 h时取出标本,测定活力谱并定量任意荧光单位(AFU)。结果:AgNPs呈不规则准球形,平均尺寸为25 nm。原子吸收光谱分析表明Ag+的溶解度较低。随着评价周期的持续至72 h,金黄色葡萄球菌生物膜的形成增加。实验组在6、12、24、48、72 h时生长较差,荧光强度的降低对金黄色葡萄球菌生物膜形成的抑制作用较对照组有统计学意义(P < 0.05)。结论:AgNPs掺入PMMA后,抑制了金黄色葡萄球菌生物膜的生长和成熟。
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Antibiofilm properties of silver nanoparticles incorporated into polymethyl methacrylate used for dental applications.

Background: Acrylic resins used in dental and biomedical applications do not have antimicrobial properties, their surface is susceptible to colonization of microorganisms.

Objective: The aim of this study was to evaluate the antibiofilm properties of silver nanoparticles (AgNPs) deposited in a polymethyl methacrylate (PMMA) surface against a Staphylococcus aureus biofilm.

Methods: The PMMA was impregnated with AgNPs by using the in-situ polymerization method. To determine the solubility of the incorporated silver (Ag+) atomic absorption spectrophotometry was used (AAS) at 24 h, 48 h, 7 days, and 30 days. Thirty specimens of PMMA with AgNPs and without NP (control group) were assembled in the CDC Biofilm Bioreactor system with a cell suspension of S. aureus. The specimens were removed at 6, 12, 24, 48, and 72 h to determine the viability profile and quantify the Arbitrary Fluorescence Units (AFU).

Results: The AgNPs showed an irregular and quasispherical shape with an average size of 25 nm. AAS analysis demonstrated a low solubility of Ag+. The formation of the S. aureus biofilm increased as the evaluation periods continued up to 72 h. The experimental group showed poor growth, and a decrease in the intensity of the fluorescence demonstrated a statistically significant inhibition of the formation of the biofilm (P < 0.05) in relation to the control group at 6, 12, 24, 48, and 72 h.

Conclusion: AgNPs incorporated into PMMA decreased the growth and maturation of S. aureus biofilm.

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来源期刊
Bio-medical materials and engineering
Bio-medical materials and engineering 工程技术-材料科学:生物材料
CiteScore
1.80
自引率
0.00%
发文量
73
审稿时长
6 months
期刊介绍: The aim of Bio-Medical Materials and Engineering is to promote the welfare of humans and to help them keep healthy. This international journal is an interdisciplinary journal that publishes original research papers, review articles and brief notes on materials and engineering for biological and medical systems. Articles in this peer-reviewed journal cover a wide range of topics, including, but not limited to: Engineering as applied to improving diagnosis, therapy, and prevention of disease and injury, and better substitutes for damaged or disabled human organs; Studies of biomaterial interactions with the human body, bio-compatibility, interfacial and interaction problems; Biomechanical behavior under biological and/or medical conditions; Mechanical and biological properties of membrane biomaterials; Cellular and tissue engineering, physiological, biophysical, biochemical bioengineering aspects; Implant failure fields and degradation of implants. Biomimetics engineering and materials including system analysis as supporter for aged people and as rehabilitation; Bioengineering and materials technology as applied to the decontamination against environmental problems; Biosensors, bioreactors, bioprocess instrumentation and control system; Application to food engineering; Standardization problems on biomaterials and related products; Assessment of reliability and safety of biomedical materials and man-machine systems; and Product liability of biomaterials and related products.
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