Zhen-Li Zhao , Shao-Hua Hu , Zhi-Sheng Wan , Wei-Zhen Bu , Song-Qiang Chen , Tian-Hong Han , Yi-Qun Lu
{"title":"icariin对体外诱导多能干细胞转化为精子细胞效率的影响。","authors":"Zhen-Li Zhao , Shao-Hua Hu , Zhi-Sheng Wan , Wei-Zhen Bu , Song-Qiang Chen , Tian-Hong Han , Yi-Qun Lu","doi":"10.1016/j.androl.2023.100373","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p>To investigate the effect of icariin on the transformation efficiency of germ cell-like cells from mouse induced pluripotent stem cells into sperm cells in vitro.</p></div><div><h3>Methods</h3><p><span>Firstly, mouse induced pluripotent stem cells were induced and cultured to transform into germ cell-like cells, and the primordial germ cell-like cells were identified by Western blot and RT-PCR. Then, different concentrations of icariin (0.1</span> <!-->μg/mL, 1<!--> <!-->μg/mL, 10<!--> <!-->μg/mL and 100<!--> <!-->μg/mL) were added into the culture medium, and the obtained primitive germ cell-like cells were cultured, Western blot and RT-PCR were used to identify the obtained sperm cells, the transformation efficiency was compared.</p></div><div><h3>Results</h3><p>The primordium germ cell-like cells obtained from mouse induced pluripotent stem cells in vitro specially expressed Oct-4 protein, C-kit protein, Mvh mRNA, Fragilis mRNA and Stella mRNA. The sperm cells were specially expressed VASA, SCP3 and γH2AX proteins. RT-PCR showed that the sperm cells were specially expressed Ddx4, Tp2 and Prm1 mRNA. Compared with the control group, the expression level of VASA protein (1.744<!--> <!-->±<!--> <!-->0.283, 2.882<!--> <!-->±<!--> <!-->0.373, 6.489<!--> <!-->±<!--> <!-->0.460), SCP3 protein (2.250<!--> <!-->±<!--> <!-->0.306, 7.058<!--> <!-->±<!--> <!-->0.521, 8.654<!--> <!-->±<!--> <!-->0.804), γH2AX protein (4.304<!--> <!-->±<!--> <!-->0.433, 5.713<!--> <!-->±<!--> <!-->0.339, 9.268<!--> <!-->±<!--> <!-->0.545), Ddx4 mRNA (1.374<!--> <!-->±<!--> <!-->0.145, 2.846<!--> <!-->±<!--> <!-->0.194, 4.021<!--> <!-->±<!--> <!-->0.154), Tp2 mRNA (1.358<!--> <!-->±<!--> <!-->0.130, 3.623<!--> <!-->±<!--> <!-->0.326, 5.811<!--> <!-->±<!--> <!-->0.390) and Prm1 mRNA (1.326<!--> <!-->±<!--> <!-->0.162, 3.487<!--> <!-->±<!--> <!-->0.237, 4.666<!--> <!-->±<!--> <!-->0.307) in 0.1<!--> <!-->μg/mL, 1<!--> <!-->μg/mL, 10<!--> <!-->μg/mL icariin experimental groups were all lower than that of VASA protein (10.560<!--> <!-->±<!--> <!-->0.413), SCP3 protein (13.804<!--> <!-->±<!--> <!-->0.642), γH2AX protein (11.874<!--> <!-->±<!--> <!-->0.464), Ddx4 mRNA (6.4005<!--> <!-->±<!--> <!-->0.361), Tp2 mRNA (7.314<!--> <!-->±<!--> <!-->0.256) and Prm1 mRNA (7.334<!--> <!-->±<!--> <!-->0.390) in 100<!--> <!-->μg/mL icariin experimental group.</p></div><div><h3>Conclusions</h3><p>Icariin can promote the transformation of mouse induced pluripotent stem cells into sperm cells in vitro, and it is concentration-dependent manner in a certain concentration range.</p></div>","PeriodicalId":49129,"journal":{"name":"Revista Internacional De Andrologia","volume":null,"pages":null},"PeriodicalIF":0.8000,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of icariin on the transformation efficiency of induced pluripotent stem cells into sperm cells in vitro\",\"authors\":\"Zhen-Li Zhao , Shao-Hua Hu , Zhi-Sheng Wan , Wei-Zhen Bu , Song-Qiang Chen , Tian-Hong Han , Yi-Qun Lu\",\"doi\":\"10.1016/j.androl.2023.100373\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><p>To investigate the effect of icariin on the transformation efficiency of germ cell-like cells from mouse induced pluripotent stem cells into sperm cells in vitro.</p></div><div><h3>Methods</h3><p><span>Firstly, mouse induced pluripotent stem cells were induced and cultured to transform into germ cell-like cells, and the primordial germ cell-like cells were identified by Western blot and RT-PCR. Then, different concentrations of icariin (0.1</span> <!-->μg/mL, 1<!--> <!-->μg/mL, 10<!--> <!-->μg/mL and 100<!--> <!-->μg/mL) were added into the culture medium, and the obtained primitive germ cell-like cells were cultured, Western blot and RT-PCR were used to identify the obtained sperm cells, the transformation efficiency was compared.</p></div><div><h3>Results</h3><p>The primordium germ cell-like cells obtained from mouse induced pluripotent stem cells in vitro specially expressed Oct-4 protein, C-kit protein, Mvh mRNA, Fragilis mRNA and Stella mRNA. The sperm cells were specially expressed VASA, SCP3 and γH2AX proteins. RT-PCR showed that the sperm cells were specially expressed Ddx4, Tp2 and Prm1 mRNA. Compared with the control group, the expression level of VASA protein (1.744<!--> <!-->±<!--> <!-->0.283, 2.882<!--> <!-->±<!--> <!-->0.373, 6.489<!--> <!-->±<!--> <!-->0.460), SCP3 protein (2.250<!--> <!-->±<!--> <!-->0.306, 7.058<!--> <!-->±<!--> <!-->0.521, 8.654<!--> <!-->±<!--> <!-->0.804), γH2AX protein (4.304<!--> <!-->±<!--> <!-->0.433, 5.713<!--> <!-->±<!--> <!-->0.339, 9.268<!--> <!-->±<!--> <!-->0.545), Ddx4 mRNA (1.374<!--> <!-->±<!--> <!-->0.145, 2.846<!--> <!-->±<!--> <!-->0.194, 4.021<!--> <!-->±<!--> <!-->0.154), Tp2 mRNA (1.358<!--> <!-->±<!--> <!-->0.130, 3.623<!--> <!-->±<!--> <!-->0.326, 5.811<!--> <!-->±<!--> <!-->0.390) and Prm1 mRNA (1.326<!--> <!-->±<!--> <!-->0.162, 3.487<!--> <!-->±<!--> <!-->0.237, 4.666<!--> <!-->±<!--> <!-->0.307) in 0.1<!--> <!-->μg/mL, 1<!--> <!-->μg/mL, 10<!--> <!-->μg/mL icariin experimental groups were all lower than that of VASA protein (10.560<!--> <!-->±<!--> <!-->0.413), SCP3 protein (13.804<!--> <!-->±<!--> <!-->0.642), γH2AX protein (11.874<!--> <!-->±<!--> <!-->0.464), Ddx4 mRNA (6.4005<!--> <!-->±<!--> <!-->0.361), Tp2 mRNA (7.314<!--> <!-->±<!--> <!-->0.256) and Prm1 mRNA (7.334<!--> <!-->±<!--> <!-->0.390) in 100<!--> <!-->μg/mL icariin experimental group.</p></div><div><h3>Conclusions</h3><p>Icariin can promote the transformation of mouse induced pluripotent stem cells into sperm cells in vitro, and it is concentration-dependent manner in a certain concentration range.</p></div>\",\"PeriodicalId\":49129,\"journal\":{\"name\":\"Revista Internacional De Andrologia\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2023-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Revista Internacional De Andrologia\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1698031X2300033X\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"ANDROLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Revista Internacional De Andrologia","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1698031X2300033X","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ANDROLOGY","Score":null,"Total":0}
Effect of icariin on the transformation efficiency of induced pluripotent stem cells into sperm cells in vitro
Objective
To investigate the effect of icariin on the transformation efficiency of germ cell-like cells from mouse induced pluripotent stem cells into sperm cells in vitro.
Methods
Firstly, mouse induced pluripotent stem cells were induced and cultured to transform into germ cell-like cells, and the primordial germ cell-like cells were identified by Western blot and RT-PCR. Then, different concentrations of icariin (0.1 μg/mL, 1 μg/mL, 10 μg/mL and 100 μg/mL) were added into the culture medium, and the obtained primitive germ cell-like cells were cultured, Western blot and RT-PCR were used to identify the obtained sperm cells, the transformation efficiency was compared.
Results
The primordium germ cell-like cells obtained from mouse induced pluripotent stem cells in vitro specially expressed Oct-4 protein, C-kit protein, Mvh mRNA, Fragilis mRNA and Stella mRNA. The sperm cells were specially expressed VASA, SCP3 and γH2AX proteins. RT-PCR showed that the sperm cells were specially expressed Ddx4, Tp2 and Prm1 mRNA. Compared with the control group, the expression level of VASA protein (1.744 ± 0.283, 2.882 ± 0.373, 6.489 ± 0.460), SCP3 protein (2.250 ± 0.306, 7.058 ± 0.521, 8.654 ± 0.804), γH2AX protein (4.304 ± 0.433, 5.713 ± 0.339, 9.268 ± 0.545), Ddx4 mRNA (1.374 ± 0.145, 2.846 ± 0.194, 4.021 ± 0.154), Tp2 mRNA (1.358 ± 0.130, 3.623 ± 0.326, 5.811 ± 0.390) and Prm1 mRNA (1.326 ± 0.162, 3.487 ± 0.237, 4.666 ± 0.307) in 0.1 μg/mL, 1 μg/mL, 10 μg/mL icariin experimental groups were all lower than that of VASA protein (10.560 ± 0.413), SCP3 protein (13.804 ± 0.642), γH2AX protein (11.874 ± 0.464), Ddx4 mRNA (6.4005 ± 0.361), Tp2 mRNA (7.314 ± 0.256) and Prm1 mRNA (7.334 ± 0.390) in 100 μg/mL icariin experimental group.
Conclusions
Icariin can promote the transformation of mouse induced pluripotent stem cells into sperm cells in vitro, and it is concentration-dependent manner in a certain concentration range.
期刊介绍：
Revista Internacional de Andrología es la revista oficial de la Asociación Española de Andrología, Medicina Sexual y Reproductiva (ASESA), la Sociedade Portuguesa de Ardrologia, la Sociedad Argentina de Andrología (SAA), la Asociación Iberoamericana de Sociedades de Andrología (ANDRO), y la Federación Española de Sociedades de Sexología.
La revista publicada trimestralmente es revisada por pares y es líder en el la especialidad y en español y portugués. Recientemente también publica artículos en inglés.
El objetivo de la revista es principalmente la promoción del conocimiento y la educación médica continua, con un enfoque especial en el público español y latinoamericano, a través de la publicación de las contribuciones importantes de la investigación en el campo. Todos los miembros de las sociedades antes mencionadas reciben la revista y otros suscriptores individuales e institucionales de España, Portugal y América Latina.
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