Irena Todorović , Danis Abrouk , Martina Kyselková , Céline Lavire , Marjolaine Rey , Vera Raičević , Jelena Jovičić-Petrović , Yvan Moënne-Loccoz , Daniel Muller
{"title":"从塞尔维亚小麦根际分离的两个新种:塞尔维亚假单胞菌和偶然血清假单胞菌。","authors":"Irena Todorović , Danis Abrouk , Martina Kyselková , Céline Lavire , Marjolaine Rey , Vera Raičević , Jelena Jovičić-Petrović , Yvan Moënne-Loccoz , Daniel Muller","doi":"10.1016/j.syapm.2023.126425","DOIUrl":null,"url":null,"abstract":"<div><p><em>Pseudomonas</em> strains IT-194P, IT-215P, IT-P366<sup>T</sup> and IT-P374<sup>T</sup> were isolated from the rhizospheres of wheat grown in soils sampled from different fields (some of them known to be disease-suppressive) located near Mionica, Serbia. Phylogenetic analysis of the 16S rRNA genes and of whole genome sequences showed that these strains belong to two potentially new species, one containing strains IT-P366<sup>T</sup> and IT-194P and clustering (whole genome analysis) next to <em>P. umsongensis</em> DSM16611<sup>T</sup>, and another species containing strains IT-P374<sup>T</sup> and IT-215P and clustering next to <em>P. koreensis</em> LMG21318<sup>T</sup>. Genome analysis confirmed the proposition of novel species, as ANI was below the threshold of 95% and dDDH below 70% for strains IT-P366<sup>T</sup> (compared with <em>P. umsongensis</em> DSM16611<sup>T</sup>) and IT-P374<sup>T</sup> (compared with <em>P. koreensis</em> LMG21318<sup>T</sup>). Unlike <em>P. umsongensis</em> DSM16611<sup>T</sup>, strains of <em>P. serbica</em> can grow on D-mannitol, but not on pectin, D-galacturonic acid, L-galactonic acid lactone and α-hydroxybutyric acid. In contrary to <em>P. koreensis</em> LMG21318<sup>T</sup>, strains of <em>P. serboccidentalis</em> can use sucrose, inosine and α-ketoglutaric acid (but not L-histidine) as carbon sources. Altogether, these results indicate the existence of two novel species for which we propose the names <em>Pseudomonas serbica</em> sp. nov., with the type strain IT-P366<sup>T</sup> (=CFBP 9060 <sup>T</sup> = LMG 32732 <sup>T</sup> = EML 1791 <sup>T</sup>) and <em>Pseudomonas serboccidentalis</em> sp. nov., with the type strain IT-P374<sup>T</sup> (=CFBP 9061 <sup>T</sup> = LMG 32734 <sup>T</sup> = EML 1792 <sup>T</sup>). Strains from this study presented a set of phytobeneficial functions modulating plant hormonal balance, plant nutrition and plant protection, suggesting a potential as Plant Growth-Promoting Rhizobacteria (PGPR).</p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"46 4","pages":"Article 126425"},"PeriodicalIF":3.3000,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Two novel species isolated from wheat rhizospheres in Serbia: Pseudomonas serbica sp. nov. and Pseudomonas serboccidentalis sp. nov.\",\"authors\":\"Irena Todorović , Danis Abrouk , Martina Kyselková , Céline Lavire , Marjolaine Rey , Vera Raičević , Jelena Jovičić-Petrović , Yvan Moënne-Loccoz , Daniel Muller\",\"doi\":\"10.1016/j.syapm.2023.126425\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><em>Pseudomonas</em> strains IT-194P, IT-215P, IT-P366<sup>T</sup> and IT-P374<sup>T</sup> were isolated from the rhizospheres of wheat grown in soils sampled from different fields (some of them known to be disease-suppressive) located near Mionica, Serbia. Phylogenetic analysis of the 16S rRNA genes and of whole genome sequences showed that these strains belong to two potentially new species, one containing strains IT-P366<sup>T</sup> and IT-194P and clustering (whole genome analysis) next to <em>P. umsongensis</em> DSM16611<sup>T</sup>, and another species containing strains IT-P374<sup>T</sup> and IT-215P and clustering next to <em>P. koreensis</em> LMG21318<sup>T</sup>. Genome analysis confirmed the proposition of novel species, as ANI was below the threshold of 95% and dDDH below 70% for strains IT-P366<sup>T</sup> (compared with <em>P. umsongensis</em> DSM16611<sup>T</sup>) and IT-P374<sup>T</sup> (compared with <em>P. koreensis</em> LMG21318<sup>T</sup>). Unlike <em>P. umsongensis</em> DSM16611<sup>T</sup>, strains of <em>P. serbica</em> can grow on D-mannitol, but not on pectin, D-galacturonic acid, L-galactonic acid lactone and α-hydroxybutyric acid. In contrary to <em>P. koreensis</em> LMG21318<sup>T</sup>, strains of <em>P. serboccidentalis</em> can use sucrose, inosine and α-ketoglutaric acid (but not L-histidine) as carbon sources. Altogether, these results indicate the existence of two novel species for which we propose the names <em>Pseudomonas serbica</em> sp. nov., with the type strain IT-P366<sup>T</sup> (=CFBP 9060 <sup>T</sup> = LMG 32732 <sup>T</sup> = EML 1791 <sup>T</sup>) and <em>Pseudomonas serboccidentalis</em> sp. nov., with the type strain IT-P374<sup>T</sup> (=CFBP 9061 <sup>T</sup> = LMG 32734 <sup>T</sup> = EML 1792 <sup>T</sup>). 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引用次数: 1
摘要
假单胞菌菌株IT-194P、IT-215P、IT-P366T和IT-P374T是从塞尔维亚Mionica附近不同农田(其中一些已知是抗病的)土壤中生长的小麦根际分离出来的。16S rRNA基因和全基因组序列的系统发育分析表明,这些菌株属于两个潜在的新种,一个包含菌株IT-P366T和IT-194P,全基因组分析结果与umsongensis DSM16611T聚类,另一个包含菌株IT-P374T和IT-215P,聚类与韩国ensis LMG21318T聚类。基因组分析证实了新物种的主张,菌株IT-P366T(与P. umsongensis DSM16611T相比)和IT-P374T(与P. koreensis LMG21318T相比)的ANI低于95%阈值,dDDH低于70%。与非sonsongensis DSM16611T不同,serbica菌株可以在d -甘露醇上生长,但不能在果胶、d -半乳糖醛酸、l -半乳糖醛酸内酯和α-羟基丁酸上生长。与韩国假单胞菌LMG21318T相反,血清巧合假单胞菌可以利用蔗糖、肌苷和α-酮戊二酸(而不是l -组氨酸)作为碳源。综上所述,本文提出了两种新菌种的存在,分别为类型菌株IT-P366T (=CFBP 9060 T = LMG 32732 T = EML 1791 T)和类型菌株IT-P374T (=CFBP 9061 T = LMG 32734 T = EML 1792 T)。该菌株具有调节植物激素平衡、植物营养和植物保护的一系列有益植物功能。提示其为植物促生长根瘤菌(Plant Growth-Promoting Rhizobacteria, PGPR)
Two novel species isolated from wheat rhizospheres in Serbia: Pseudomonas serbica sp. nov. and Pseudomonas serboccidentalis sp. nov.
Pseudomonas strains IT-194P, IT-215P, IT-P366T and IT-P374T were isolated from the rhizospheres of wheat grown in soils sampled from different fields (some of them known to be disease-suppressive) located near Mionica, Serbia. Phylogenetic analysis of the 16S rRNA genes and of whole genome sequences showed that these strains belong to two potentially new species, one containing strains IT-P366T and IT-194P and clustering (whole genome analysis) next to P. umsongensis DSM16611T, and another species containing strains IT-P374T and IT-215P and clustering next to P. koreensis LMG21318T. Genome analysis confirmed the proposition of novel species, as ANI was below the threshold of 95% and dDDH below 70% for strains IT-P366T (compared with P. umsongensis DSM16611T) and IT-P374T (compared with P. koreensis LMG21318T). Unlike P. umsongensis DSM16611T, strains of P. serbica can grow on D-mannitol, but not on pectin, D-galacturonic acid, L-galactonic acid lactone and α-hydroxybutyric acid. In contrary to P. koreensis LMG21318T, strains of P. serboccidentalis can use sucrose, inosine and α-ketoglutaric acid (but not L-histidine) as carbon sources. Altogether, these results indicate the existence of two novel species for which we propose the names Pseudomonas serbica sp. nov., with the type strain IT-P366T (=CFBP 9060 T = LMG 32732 T = EML 1791 T) and Pseudomonas serboccidentalis sp. nov., with the type strain IT-P374T (=CFBP 9061 T = LMG 32734 T = EML 1792 T). Strains from this study presented a set of phytobeneficial functions modulating plant hormonal balance, plant nutrition and plant protection, suggesting a potential as Plant Growth-Promoting Rhizobacteria (PGPR).
期刊介绍:
Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology: