组织发育中的LncRNA功能筛选

IF 3.6 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Non-Coding RNA Pub Date : 2023-06-28 DOI:10.3390/ncrna9040036
Yang Li, Huicong Zhai, Lingxiu Tong, Cuicui Wang, Zhiming Xie, Ke Zheng
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引用次数: 0

摘要

尽管长链非编码rna (lncRNAs)在各种细胞和生物体中被广泛研究,但关于其功能流行的争议仍在继续。在动物中,lncrna引起了普遍的兴趣,因为它们的转录组谱呈指数增长,报道了它们高度组织特异性和发育动态的表达,更重要的是,越来越多的实验证据支持它们在促进器官发生和个体适应性方面的功能。在哺乳动物睾丸中,虽然鉴定了大量的lncRNA物种,但迄今为止,只有少数lncRNA物种被证明是有用的,甚至更少的lncRNA物种被证明是男性生育能力的真正要求。这种明显的差距归因于大量垃圾lncrna的实际存在,缺乏理想的种系培养系统,难以进行功能丧失的询问,以及有限的筛选策略。面对这些挑战,在这篇综述中,我们讨论了lncRNA在机体发育中的功能,特别是在小鼠睾丸中的功能,重点是lncRNA的功能筛选。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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LncRNA Functional Screening in Organismal Development.

Controversy continues over the functional prevalence of long non-coding RNAs (lncRNAs) despite their being widely investigated in all kinds of cells and organisms. In animals, lncRNAs have aroused general interest from exponentially increasing transcriptomic repertoires reporting their highly tissue-specific and developmentally dynamic expression, and more importantly, from growing experimental evidence supporting their functionality in facilitating organogenesis and individual fitness. In mammalian testes, while a great multitude of lncRNA species are identified, only a minority of them have been shown to be useful, and even fewer have been demonstrated as true requirements for male fertility using knockout models to date. This noticeable gap is attributed to the virtual existence of a large number of junk lncRNAs, the lack of an ideal germline culture system, difficulty in loss-of-function interrogation, and limited screening strategies. Facing these challenges, in this review, we discuss lncRNA functionality in organismal development and especially in mouse testis, with a focus on lncRNAs with functional screening.

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来源期刊
Non-Coding RNA
Non-Coding RNA Biochemistry, Genetics and Molecular Biology-Genetics
CiteScore
6.70
自引率
4.70%
发文量
74
审稿时长
10 weeks
期刊介绍: Functional studies dealing with identification, structure-function relationships or biological activity of: small regulatory RNAs (miRNAs, siRNAs and piRNAs) associated with the RNA interference pathway small nuclear RNAs, small nucleolar and tRNAs derived small RNAs other types of small RNAs, such as those associated with splice junctions and transcription start sites long non-coding RNAs, including antisense RNAs, long ''intergenic'' RNAs, intronic RNAs and ''enhancer'' RNAs other classes of RNAs such as vault RNAs, scaRNAs, circular RNAs, 7SL RNAs, telomeric and centromeric RNAs regulatory functions of mRNAs and UTR-derived RNAs catalytic and allosteric (riboswitch) RNAs viral, transposon and repeat-derived RNAs bacterial regulatory RNAs, including CRISPR RNAS Analysis of RNA processing, RNA binding proteins, RNA signaling and RNA interaction pathways: DICER AGO, PIWI and PIWI-like proteins other classes of RNA binding and RNA transport proteins RNA interactions with chromatin-modifying complexes RNA interactions with DNA and other RNAs the role of RNA in the formation and function of specialized subnuclear organelles and other aspects of cell biology intercellular and intergenerational RNA signaling RNA processing structure-function relationships in RNA complexes RNA analyses, informatics, tools and technologies: transcriptomic analyses and technologies development of tools and technologies for RNA biology and therapeutics Translational studies involving long and short non-coding RNAs: identification of biomarkers development of new therapies involving microRNAs and other ncRNAs clinical studies involving microRNAs and other ncRNAs.
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