{"title":"液相色谱-串联质谱法定量测定鸡血浆中Nτ -甲基组氨酸和n π-甲基组氨酸。","authors":"Jun-Ichi Shiraishi, Daichi Ijiri, Ayumi Katafuchi, Shozo Tomonaga, Saki Shimamoto, Hanwool Do, Shinya Ishihara, Akira Ohtsuka","doi":"10.2141/jpsa.2023017","DOIUrl":null,"url":null,"abstract":"<p><p>The concentration of <i>N</i><sup>τ</sup>-methylhistidine in plasma provides an index of skeletal muscle protein breakdown. This study aimed to establish a quantitative method for measuring the concentrations of <i>N</i><sup>τ</sup>-methylhistidine and its isomer <i>N</i><sup>π</sup>-methylhistidine in chicken plasma, using liquid chromatography-tandem mass spectrometry with stable isotope dilution analysis. The acceptable linear ranges of detection were 1.56-50.00 μmol/L for <i>N</i><sup>τ</sup>-methylhistidine and 0.78-25.00 μmol/L for <i>N</i><sup>π</sup>-methylhistidine. The proposed method detected changes in the plasma levels of <i>N</i><sup>τ</sup>-methylhistidine and <i>N</i><sup>π</sup>-methylhistidine in response to fasting and re-feeding. These results suggest that the method developed in this study can be used for the simultaneous measurement of <i>N</i><sup>τ</sup>-methylhistidine and <i>N</i><sup>π</sup>-methylhistidine in chicken plasma.</p>","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"60 ","pages":"2023017"},"PeriodicalIF":1.8000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/35/e1/jpsa-60-2023017.PMC10357029.pdf","citationCount":"1","resultStr":"{\"title\":\"Quantification of N<sup>τ</sup> -Methylhistidine and N<sup>π</sup>-Methylhistidine in Chicken Plasma by Liquid Chromatography-Tandem Mass Spectrometry.\",\"authors\":\"Jun-Ichi Shiraishi, Daichi Ijiri, Ayumi Katafuchi, Shozo Tomonaga, Saki Shimamoto, Hanwool Do, Shinya Ishihara, Akira Ohtsuka\",\"doi\":\"10.2141/jpsa.2023017\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The concentration of <i>N</i><sup>τ</sup>-methylhistidine in plasma provides an index of skeletal muscle protein breakdown. This study aimed to establish a quantitative method for measuring the concentrations of <i>N</i><sup>τ</sup>-methylhistidine and its isomer <i>N</i><sup>π</sup>-methylhistidine in chicken plasma, using liquid chromatography-tandem mass spectrometry with stable isotope dilution analysis. The acceptable linear ranges of detection were 1.56-50.00 μmol/L for <i>N</i><sup>τ</sup>-methylhistidine and 0.78-25.00 μmol/L for <i>N</i><sup>π</sup>-methylhistidine. The proposed method detected changes in the plasma levels of <i>N</i><sup>τ</sup>-methylhistidine and <i>N</i><sup>π</sup>-methylhistidine in response to fasting and re-feeding. These results suggest that the method developed in this study can be used for the simultaneous measurement of <i>N</i><sup>τ</sup>-methylhistidine and <i>N</i><sup>π</sup>-methylhistidine in chicken plasma.</p>\",\"PeriodicalId\":16883,\"journal\":{\"name\":\"Journal of Poultry Science\",\"volume\":\"60 \",\"pages\":\"2023017\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/35/e1/jpsa-60-2023017.PMC10357029.pdf\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Poultry Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.2141/jpsa.2023017\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Poultry Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.2141/jpsa.2023017","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
Quantification of Nτ -Methylhistidine and Nπ-Methylhistidine in Chicken Plasma by Liquid Chromatography-Tandem Mass Spectrometry.
The concentration of Nτ-methylhistidine in plasma provides an index of skeletal muscle protein breakdown. This study aimed to establish a quantitative method for measuring the concentrations of Nτ-methylhistidine and its isomer Nπ-methylhistidine in chicken plasma, using liquid chromatography-tandem mass spectrometry with stable isotope dilution analysis. The acceptable linear ranges of detection were 1.56-50.00 μmol/L for Nτ-methylhistidine and 0.78-25.00 μmol/L for Nπ-methylhistidine. The proposed method detected changes in the plasma levels of Nτ-methylhistidine and Nπ-methylhistidine in response to fasting and re-feeding. These results suggest that the method developed in this study can be used for the simultaneous measurement of Nτ-methylhistidine and Nπ-methylhistidine in chicken plasma.
期刊介绍：
The Journal of Poultry Science will publish original reports and reviews which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: breeding and genetics, nutrition and feeds, physiology, reproduction, immunology, behavior, environmental science, management and housing welfare, processing and products, and health in poultry. Submission of original articles to the Journal is open to all poultry researchers. The review articles are invited papers written by international outstanding researchers. Articles will be published in English, American style.
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