[约尔氏疟原虫感染小鼠肺部TIGIT+CD8+ T细胞数量增加,但细胞因子分泌减少]。

Anqi Xie, Jiajie Li, Chao Fang, Feihu Shi, Junmin Xing, Feng Mo, Hongyan Xie, Jun Huang, Haixia Wei
{"title":"[约尔氏疟原虫感染小鼠肺部TIGIT+CD8+ T细胞数量增加,但细胞因子分泌减少]。","authors":"Anqi Xie,&nbsp;Jiajie Li,&nbsp;Chao Fang,&nbsp;Feihu Shi,&nbsp;Junmin Xing,&nbsp;Feng Mo,&nbsp;Hongyan Xie,&nbsp;Jun Huang,&nbsp;Haixia Wei","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Objective To investigate the effect of T cell immunoreceptor with Ig and ITIM domains (TIGIT) on the function of CD8<sup>+</sup> T cells in the lungs of Plasmodium infected mice. Methods The lungs of the mice infected with Plasmodium yoelii were isolated, weighed and photographed after 12 days' infection. After dissolution, lung lymphocytes were isolated, counted and stained, and then the contents of CD8<sup>+</sup> and TIGIT<sup>+</sup>CD8<sup>+</sup> T cells were detected by flow cytometry. The expressions of L selectin (CD62L), CD69, programmed death 1 (PD-1), CD25, and C-X3-C motif chemokine receptor 1 (CX3CR1) on TIGIT<sup>+</sup>CD8<sup>+</sup> T cells were detected by flow cytometry. After stimulation with phorbol 12-myristate 13-acetate (PMA) and ionomycin, the ability of TIGIT<sup>+</sup>CD8<sup>+</sup>T cells to secrete interferon γ(IFN-γ), interleukin 21 (IL-21), IL-4, IL-17, and IL-10 was detected. Results The body mass of mice with Plasmodium infection was reduced. The lungs became darker, and the ratio of the lung mass to body mass was significantly increased. Compared with the normal mice, the percentages and absolute quantity of CD8<sup>+</sup> and TIGIT<sup>+</sup>CD8<sup>+</sup> T cells in the lungs of the infected mice were significantly increased. The percentage of TIGIT<sup>+</sup>CD8<sup>+</sup> T cells expressing CD62L in the infected group was significantly lower, while the percentage of the CD69, PD-1, and CX3CR1 cells were significantly higher than that of TIGIT<sup>+</sup>CD8<sup>+</sup> T cells from the normal mice. The percentages of TIGIT<sup>+</sup>CD8<sup>+</sup> T cells secreting IL-21, IL-4, IL-17 and IL-10 cells in the infected group were significantly lower. Conclusion The lung lesions from mice with Plasmodium infection are obvious, the numbers of TIGIT<sup>+</sup>CD8<sup>+</sup> T cells increase, and these cells express a variety of activation-related molecules, but the ability to secrete cytokines is reduced.</p>","PeriodicalId":23737,"journal":{"name":"Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology","volume":"39 8","pages":"673-679"},"PeriodicalIF":0.0000,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[The number of TIGIT<sup>+</sup>CD8<sup>+</sup> T cells increases but their cytokine secretion decreases in the lungs of Plasmodium yoelii infected mice].\",\"authors\":\"Anqi Xie,&nbsp;Jiajie Li,&nbsp;Chao Fang,&nbsp;Feihu Shi,&nbsp;Junmin Xing,&nbsp;Feng Mo,&nbsp;Hongyan Xie,&nbsp;Jun Huang,&nbsp;Haixia Wei\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Objective To investigate the effect of T cell immunoreceptor with Ig and ITIM domains (TIGIT) on the function of CD8<sup>+</sup> T cells in the lungs of Plasmodium infected mice. Methods The lungs of the mice infected with Plasmodium yoelii were isolated, weighed and photographed after 12 days' infection. After dissolution, lung lymphocytes were isolated, counted and stained, and then the contents of CD8<sup>+</sup> and TIGIT<sup>+</sup>CD8<sup>+</sup> T cells were detected by flow cytometry. The expressions of L selectin (CD62L), CD69, programmed death 1 (PD-1), CD25, and C-X3-C motif chemokine receptor 1 (CX3CR1) on TIGIT<sup>+</sup>CD8<sup>+</sup> T cells were detected by flow cytometry. After stimulation with phorbol 12-myristate 13-acetate (PMA) and ionomycin, the ability of TIGIT<sup>+</sup>CD8<sup>+</sup>T cells to secrete interferon γ(IFN-γ), interleukin 21 (IL-21), IL-4, IL-17, and IL-10 was detected. Results The body mass of mice with Plasmodium infection was reduced. The lungs became darker, and the ratio of the lung mass to body mass was significantly increased. Compared with the normal mice, the percentages and absolute quantity of CD8<sup>+</sup> and TIGIT<sup>+</sup>CD8<sup>+</sup> T cells in the lungs of the infected mice were significantly increased. The percentage of TIGIT<sup>+</sup>CD8<sup>+</sup> T cells expressing CD62L in the infected group was significantly lower, while the percentage of the CD69, PD-1, and CX3CR1 cells were significantly higher than that of TIGIT<sup>+</sup>CD8<sup>+</sup> T cells from the normal mice. The percentages of TIGIT<sup>+</sup>CD8<sup>+</sup> T cells secreting IL-21, IL-4, IL-17 and IL-10 cells in the infected group were significantly lower. Conclusion The lung lesions from mice with Plasmodium infection are obvious, the numbers of TIGIT<sup>+</sup>CD8<sup>+</sup> T cells increase, and these cells express a variety of activation-related molecules, but the ability to secrete cytokines is reduced.</p>\",\"PeriodicalId\":23737,\"journal\":{\"name\":\"Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology\",\"volume\":\"39 8\",\"pages\":\"673-679\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

目的探讨含Ig和ITIM结构域的T细胞免疫受体(TIGIT)对疟原虫感染小鼠肺部CD8+ T细胞功能的影响。方法对感染约氏疟原虫的小鼠进行肺分离,感染12 d后称重拍照。溶解后,分离肺淋巴细胞,计数染色,流式细胞术检测CD8+和TIGIT+CD8+ T细胞的含量。流式细胞术检测L选择素(CD62L)、CD69、程序性死亡1 (PD-1)、CD25、C-X3-C基序趋化因子受体1 (CX3CR1)在TIGIT+CD8+ T细胞上的表达。在12-肉豆酸酯(PMA)和离子霉素刺激后,检测TIGIT+CD8+T细胞分泌干扰素γ(IFN-γ)、白细胞介素21 (IL-21)、IL-4、IL-17和IL-10的能力。结果小鼠感染疟原虫后体重明显下降。肺变暗,肺质量与体重之比明显增加。与正常小鼠相比,感染小鼠肺中CD8+和TIGIT+CD8+ T细胞的百分比和绝对数量均显著增加。感染组TIGIT+CD8+ T细胞表达CD62L的比例显著降低,而CD69、PD-1和CX3CR1细胞的比例显著高于正常小鼠的TIGIT+CD8+ T细胞。感染组TIGIT+CD8+ T细胞分泌IL-21、IL-4、IL-17、IL-10细胞的比例显著降低。结论疟原虫感染小鼠肺部病变明显,TIGIT+CD8+ T细胞数量增加,表达多种活化相关分子,但分泌细胞因子的能力降低。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
[The number of TIGIT+CD8+ T cells increases but their cytokine secretion decreases in the lungs of Plasmodium yoelii infected mice].

Objective To investigate the effect of T cell immunoreceptor with Ig and ITIM domains (TIGIT) on the function of CD8+ T cells in the lungs of Plasmodium infected mice. Methods The lungs of the mice infected with Plasmodium yoelii were isolated, weighed and photographed after 12 days' infection. After dissolution, lung lymphocytes were isolated, counted and stained, and then the contents of CD8+ and TIGIT+CD8+ T cells were detected by flow cytometry. The expressions of L selectin (CD62L), CD69, programmed death 1 (PD-1), CD25, and C-X3-C motif chemokine receptor 1 (CX3CR1) on TIGIT+CD8+ T cells were detected by flow cytometry. After stimulation with phorbol 12-myristate 13-acetate (PMA) and ionomycin, the ability of TIGIT+CD8+T cells to secrete interferon γ(IFN-γ), interleukin 21 (IL-21), IL-4, IL-17, and IL-10 was detected. Results The body mass of mice with Plasmodium infection was reduced. The lungs became darker, and the ratio of the lung mass to body mass was significantly increased. Compared with the normal mice, the percentages and absolute quantity of CD8+ and TIGIT+CD8+ T cells in the lungs of the infected mice were significantly increased. The percentage of TIGIT+CD8+ T cells expressing CD62L in the infected group was significantly lower, while the percentage of the CD69, PD-1, and CX3CR1 cells were significantly higher than that of TIGIT+CD8+ T cells from the normal mice. The percentages of TIGIT+CD8+ T cells secreting IL-21, IL-4, IL-17 and IL-10 cells in the infected group were significantly lower. Conclusion The lung lesions from mice with Plasmodium infection are obvious, the numbers of TIGIT+CD8+ T cells increase, and these cells express a variety of activation-related molecules, but the ability to secrete cytokines is reduced.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
[miR-18a ameliorates inflammation and tissue injury in a mouse model of allergic rhinitis via blocking TLR4/NF-κB pathway]. [The number of TIGIT+CD8+ T cells increases but their cytokine secretion decreases in the lungs of Plasmodium yoelii infected mice]. [The mechanism of microcystin leucine-arginine (MC-LR)-induced injury of Sertoli cell immune response and biological behavior]. [IgG Fc binding protein (FCGBP) as a prognostic marker of low-grade glioma and its correlation analysis with immune infiltration]. [Sinomenine ameliorates bleomycin A5-induced pulmonary fibrosis by blocking the miR-21/ADAMTS-1 signaling pathway in rats].
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1