一种新的细胞学染色方法:甲状腺细针穿刺细胞学快速现场评价的快速方法。

IF 1.6 4区 医学 Q3 PATHOLOGY Acta Cytologica Pub Date : 2023-01-01 DOI:10.1159/000527347
Paula de Melo Alves, Fernando Ferreira, Telma Oliveira, Daniela Alves, Sule Canberk, Fernando C Schmitt
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引用次数: 0

摘要

简介:甲状腺细针穿刺(FNA)在细胞学文献中是一种成熟的技术。通过在细胞学实践中引入快速染色,快速现场评估(ROSE)的不断增加的效用加强了FNA作为患者管理的主要诊断方法的地位。市场上有几种ROSE染色剂可供选择,即diff - quick (DQ)、甲苯胺蓝和超快Papanicolaou染色剂。最近,我们的团队开发了一种新的染色变体,标记为原始的“BlueStain®”技术,以前没有在这种情况下进行过测试。方法:对40例FNA甲状腺病例进行分析。每位患者至少准备两张载玻片:一张用DQ染色,另一张用BlueStain®染色。同时,通过比较两种染色方法,进行ROSE诊断,评估背景、细胞度、胶体存在细节、细胞形态、核细节、细胞质细节和整体染色等参数,分别以1到3分进行评分,分别代表差、平均和良好。结果:BlueStain®的质量指数略好(53%对47%),但两种染色剂之间无显著差异。BlueStain®在胶体的存在和类型以及核细节方面提供了更好的细节,这对于甲状腺细胞学诊断非常重要。对同一患者的两种染色进行比较,有8例诊断不一致。在5例不确定病例中,BlueStain®允许将其带入良性类别,可能是因为这种染色方法可以清楚地观察到涂片背景中的胶体。然而,由于我们观察的是两种不同的载玻片,我们不能排除这些差异是样品收集和/或涂抹的问题。结论:我们的数据表明,BlueStain®适合为ROSE研究的甲状腺吸入物的初步评估提供高质量的载玻片。事实上,在某些方面,这种新的染色方法可以更好地保存胶体和细胞细节,显示出它是DQ染色变体的替代品,在保持性能水平的同时便宜10倍,更简单,因为它只需要一个染色步骤。
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A New Cytology Staining Method: A Fast Approach for Rapid On-Site Evaluation on Thyroid Fine-Needle Aspiration Cytology.

Introduction: Thyroid fine-needle aspiration (FNA) is a well-established technique in the cytology literature. Through the introduction of rapid stains in cytology practice, the ever-increasing utility of rapid on-site evaluation (ROSE) has strengthened the place of FNA as a primary diagnostic method in patient management. There are few stain variants available in the market for ROSE, namely Diff-Quik (DQ), Toluidine blue, and ultrafast Papanicolaou stains. Recently, our group developed a new staining variant labeled as original "BlueStain®" technique that was not previously tested in this context.

Methods: 40 FNA thyroid cases were studied. At least two slides were prepared from each patient: one stained by DQ and the other by BlueStain®. Simultaneously, a ROSE diagnosis was performed as the two staining methods were compared, evaluating the parameters of background, cellularity, details of colloid presence, cell morphology, nuclear details, cytoplasmic details, and overall staining, scored on a scale from 1 to 3, representing poor, average, and good, respectively.

Results: The quality index was slightly better for BlueStain® (53% vs. 47%) but not significantly different between the two stains. BlueStain® provides better details in both the presence and type of colloid as well as nuclear details, which are regarded as very important for diagnosis in thyroid cytology. There were eight cases with discordant diagnosis when compared between two stains from the same patient. In five cases of indeterminate cases, BlueStain® allows to bring them to the benign category, probably because this staining method allows a clear observation of the colloid in the background of the smears. However, since we are observing two different slides, we cannot rule out that these differences are a question of sample collecting and/or smearing.

Conclusions: Our data demonstrates that BlueStain® is suitable to provide good-quality slides for primary assessments of thyroid aspirates studied by ROSE. In fact, in some aspects, this new staining method shows better preservation of colloid and cell details, revealing itself as an alternative to the DQ stain variant, upholding performance level while being 10 times cheaper and simpler because it requires just one step of staining.

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来源期刊
Acta Cytologica
Acta Cytologica 生物-病理学
CiteScore
3.70
自引率
11.10%
发文量
46
审稿时长
4-8 weeks
期刊介绍: With articles offering an excellent balance between clinical cytology and cytopathology, ''Acta Cytologica'' fosters the understanding of the pathogenetic mechanisms behind cytomorphology and thus facilitates the translation of frontline research into clinical practice. As the official journal of the International Academy of Cytology and affiliated to over 50 national cytology societies around the world, ''Acta Cytologica'' evaluates new and existing diagnostic applications of scientific advances as well as their clinical correlations. Original papers, review articles, meta-analyses, novel insights from clinical practice, and letters to the editor cover topics from diagnostic cytopathology, gynecologic and non-gynecologic cytopathology to fine needle aspiration, molecular techniques and their diagnostic applications. As the perfect reference for practical use, ''Acta Cytologica'' addresses a multidisciplinary audience practicing clinical cytopathology, cell biology, oncology, interventional radiology, otorhinolaryngology, gastroenterology, urology, pulmonology and preventive medicine.
期刊最新文献
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