{"title":"E-钙粘蛋白和β-儿茶素在口腔鳞状细胞癌中的免疫组织化学表达。","authors":"Akindayo Olufunto Akinyamoju, Ahmed Oluwatoyin Lawal, Akinyele Olumuyiwa Adisa, Bukola Folasade Adeyemi, Bamidele Kolude","doi":"10.4103/jwas.jwas_1_23","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The E-cadherin/β-catenin protein complexes are actively involved in the epithelial-to-mesenchymal transition. Alterations in cadherin or catenin expression or function, play important roles in the development of invasive or metastatic phenotypes of cancers.</p><p><strong>Objectives: </strong>The aim of this study was to assess the expression of E-cadherin and β-catenin in oral squamous cell carcinoma (OSCC) patients and to compare this with their clinico-pathological parameters.</p><p><strong>Materials and methods: </strong>This was a cross-sectional study to assess the immunohistochemical expression of E-cadherin and β-catenin in 41 cases of OSCC. Data were analyzed using version 26 of SPSS software. Qualitative data were compared using chi-square statistics. Quantitative data were summarized using mean, standard deviation, and confidence interval and compared using a one-way analysis of variance test. The level of significance was set at <i>P</i> < 0.05.</p><p><strong>Results: </strong>Overall, 95.1% of the cases had positive membrane expression for E-cadherin, while cytoplasmic staining was seen in 90.2% cases. Positive nuclear staining was seen in 46.3% cases. There was a decrease in the percentage of cytoplasmic and nuclear expression of E-cadherin as the OSCC became more poorly differentiated (χ<sup>2</sup> = 13.96, <i>P</i> = 0.016). Also, a decrease in the percentage of nuclear expression of β-catenin in poorly differentiated cases was seen. However, no statistically significant difference was seen in the expression of β-catenin between the different histologic grades (χ<sup>2</sup> = 4.8, <i>P</i> = 0.4).</p><p><strong>Conclusion: </strong>This study shows a reduction in the expression of E-cadherin and β-catenin as OSCC becomes less differentiated.</p>","PeriodicalId":73993,"journal":{"name":"Journal of the West African College of Surgeons","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/24/2b/JWACS-13-43.PMC10395847.pdf","citationCount":"1","resultStr":"{\"title\":\"Immunohistochemical Expression of E-Cadherin and β-Catenin in Oral Squamous Cell Carcinoma.\",\"authors\":\"Akindayo Olufunto Akinyamoju, Ahmed Oluwatoyin Lawal, Akinyele Olumuyiwa Adisa, Bukola Folasade Adeyemi, Bamidele Kolude\",\"doi\":\"10.4103/jwas.jwas_1_23\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>The E-cadherin/β-catenin protein complexes are actively involved in the epithelial-to-mesenchymal transition. Alterations in cadherin or catenin expression or function, play important roles in the development of invasive or metastatic phenotypes of cancers.</p><p><strong>Objectives: </strong>The aim of this study was to assess the expression of E-cadherin and β-catenin in oral squamous cell carcinoma (OSCC) patients and to compare this with their clinico-pathological parameters.</p><p><strong>Materials and methods: </strong>This was a cross-sectional study to assess the immunohistochemical expression of E-cadherin and β-catenin in 41 cases of OSCC. Data were analyzed using version 26 of SPSS software. Qualitative data were compared using chi-square statistics. Quantitative data were summarized using mean, standard deviation, and confidence interval and compared using a one-way analysis of variance test. The level of significance was set at <i>P</i> < 0.05.</p><p><strong>Results: </strong>Overall, 95.1% of the cases had positive membrane expression for E-cadherin, while cytoplasmic staining was seen in 90.2% cases. Positive nuclear staining was seen in 46.3% cases. There was a decrease in the percentage of cytoplasmic and nuclear expression of E-cadherin as the OSCC became more poorly differentiated (χ<sup>2</sup> = 13.96, <i>P</i> = 0.016). Also, a decrease in the percentage of nuclear expression of β-catenin in poorly differentiated cases was seen. However, no statistically significant difference was seen in the expression of β-catenin between the different histologic grades (χ<sup>2</sup> = 4.8, <i>P</i> = 0.4).</p><p><strong>Conclusion: </strong>This study shows a reduction in the expression of E-cadherin and β-catenin as OSCC becomes less differentiated.</p>\",\"PeriodicalId\":73993,\"journal\":{\"name\":\"Journal of the West African College of Surgeons\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/24/2b/JWACS-13-43.PMC10395847.pdf\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of the West African College of Surgeons\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4103/jwas.jwas_1_23\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/6/27 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the West African College of Surgeons","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/jwas.jwas_1_23","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/6/27 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
Immunohistochemical Expression of E-Cadherin and β-Catenin in Oral Squamous Cell Carcinoma.
Background: The E-cadherin/β-catenin protein complexes are actively involved in the epithelial-to-mesenchymal transition. Alterations in cadherin or catenin expression or function, play important roles in the development of invasive or metastatic phenotypes of cancers.
Objectives: The aim of this study was to assess the expression of E-cadherin and β-catenin in oral squamous cell carcinoma (OSCC) patients and to compare this with their clinico-pathological parameters.
Materials and methods: This was a cross-sectional study to assess the immunohistochemical expression of E-cadherin and β-catenin in 41 cases of OSCC. Data were analyzed using version 26 of SPSS software. Qualitative data were compared using chi-square statistics. Quantitative data were summarized using mean, standard deviation, and confidence interval and compared using a one-way analysis of variance test. The level of significance was set at P < 0.05.
Results: Overall, 95.1% of the cases had positive membrane expression for E-cadherin, while cytoplasmic staining was seen in 90.2% cases. Positive nuclear staining was seen in 46.3% cases. There was a decrease in the percentage of cytoplasmic and nuclear expression of E-cadherin as the OSCC became more poorly differentiated (χ2 = 13.96, P = 0.016). Also, a decrease in the percentage of nuclear expression of β-catenin in poorly differentiated cases was seen. However, no statistically significant difference was seen in the expression of β-catenin between the different histologic grades (χ2 = 4.8, P = 0.4).
Conclusion: This study shows a reduction in the expression of E-cadherin and β-catenin as OSCC becomes less differentiated.