微小RNA作为马间充质基质细胞软骨分化潜能的预后标志物。

IF 2.5 3区 医学 Q3 CELL & TISSUE ENGINEERING Stem cells and development Pub Date : 2023-11-01 Epub Date: 2023-09-27 DOI:10.1089/scd.2022.0295
A Hamed Alizadeh, Starlee Lively, Sarah Lepage, Pratibha Potla, Stewart Russell, Shabana Amanda Ali, Mohit Kapoor, Thomas G Koch
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引用次数: 0

摘要

间充质基质细胞(MSCs)是动物和人类软骨组织再生的一种很有前途的细胞来源,但其体外软骨分化潜力存在很大的供体间差异。导致培养扩展MSC异质性的潜在分子机制仍知之甚少。在这项研究中,我们试图确定与不同供体培养的马MSC软骨分化潜力相关的微小RNA(miRNA)特征的变化。根据马脐带血来源的MSCs的组织学外观和糖胺聚糖沉积的定量,将其产生的新软骨组织分为具有高或低软骨生成潜力(LCP)。使用下一代测序,我们在未分化的MSC培养物中鉴定了30种差异表达的miRNA,这些miRNA与其软骨形成潜力相对应。值得注意的是,具有LCP的MSCs上调miR-146a和miR-487b-3p,这也通过定量实时聚合酶链反应观察到。我们的研究结果表明,马MSC培养物的miRNA图谱可能对选择MSC供体的软骨分化潜力具有预后价值。
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MicroRNAs as Prognostic Markers for Chondrogenic Differentiation Potential of Equine Mesenchymal Stromal Cells.

Mesenchymal stromal cells (MSCs) are a promising cell source for cartilage tissue regeneration in animals and humans but with large interdonor variation in their in vitro chondrogenic differentiation potential. Underlying molecular mechanisms responsible for culture-expanded MSC heterogeneity remain poorly understood. In this study, we sought to identify variations in microRNA (miRNA) signatures associated with cultured equine MSC chondrogenic differentiation potential from different donors. Neocartilage tissue generated from equine cord blood-derived MSCs was categorized as having either high or low chondrogenic potential (LCP) based on their histological appearance and quantification of glycosaminoglycan deposition. Using next-generation sequencing, we identified 30 differentially expressed miRNAs among undifferentiated MSC cultures that corresponded with their chondrogenic potential. Of note, MSCs with LCP upregulated miR-146a and miR-487b-3p, which was also observed by quantitative real-time polymerase chain reaction. Our findings suggest that miRNA profiling of equine MSC cultures may have prognostic value in selecting MSC donors with regard to their chondrogenic differentiation potential.

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来源期刊
Stem cells and development
Stem cells and development 医学-细胞与组织工程
CiteScore
7.80
自引率
2.50%
发文量
69
审稿时长
3 months
期刊介绍: Stem Cells and Development is globally recognized as the trusted source for critical, even controversial coverage of emerging hypotheses and novel findings. With a focus on stem cells of all tissue types and their potential therapeutic applications, the Journal provides clinical, basic, and translational scientists with cutting-edge research and findings. Stem Cells and Development coverage includes: Embryogenesis and adult counterparts of this process Physical processes linking stem cells, primary cell function, and structural development Hypotheses exploring the relationship between genotype and phenotype Development of vasculature, CNS, and other germ layer development and defects Pluripotentiality of embryonic and somatic stem cells The role of genetic and epigenetic factors in development
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