Disruption of a DNA fragment that encodes the microneme adhesive repeat domain-containing region of the BBOV_III011730 does not affect the blood stage growth of Babesia bovis in vitro

IF 1.4 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular and biochemical parasitology Pub Date : 2023-09-01 DOI:10.1016/j.molbiopara.2023.111576

Bumduuren Tuvshintulga , Azirwan Guswanto, Arifin Budiman Nugraha , Thillaiampalam Sivakumar, Rika Umemiya-Shirafuji, Naoaki Yokoyama

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Abstract

Babesia bovis, an intraerythrocytic hemoprotozoan parasite, causes the most pathogenic form of bovine babesiosis, negatively impacting the cattle industry. Comprehensive knowledge of B. bovis biology is necessary for developing control methods. In cattle, B. bovis invades the red blood cells (RBCs) and reproduces asexually. Micronemal proteins, which bind to sialic acid of host cells via their microneme adhesive repeat (MAR) domains, are believed to play a key role in host cell invasion by apicomplexan parasites. In this study, we successfully deleted the region encoding MAR domain of the BBOV_III011730 by integrating a fusion gene of enhanced green fluorescent protein-blasticidin-S-deaminase into the genome of B. bovis. The transgenic B. bovis, lacking the MAR domain of the BBOV_III011730, invaded bovine RBCs in vitro and grew at rates similar to the parental line. In conclusion, our study revealed that the MAR domain is non-essential for the intraerythrocytic development of B. bovis in vitro.

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编码BBOV_III011730的含有微丝粘附重复结构域的区域的DNA片段的破坏不会影响体外牛巴贝斯虫的血液期生长

牛巴贝斯虫是一种红细胞内的血液原体寄生虫，引起最具致病性的牛巴贝斯病，对畜牧业产生负面影响。对牛双歧杆菌生物学的全面了解对于开发控制方法是必要的。在牛身上，牛双歧杆菌侵入红细胞并进行无性繁殖。微核蛋白通过其微核粘附重复序列（MAR）结构域与宿主细胞的唾液酸结合，被认为在顶复门寄生虫入侵宿主细胞中起着关键作用。在本研究中，我们通过将增强型绿色荧光蛋白-blasticin-S-deaminase的融合基因整合到牛双歧杆菌基因组中，成功删除了BBOV_III011730的MAR结构域编码区。缺乏BBOV_III011730的MAR结构域的转基因牛双歧杆菌在体外侵入牛RBCs，并以与亲本系相似的速率生长。总之，我们的研究表明，MAR结构域对体外牛双歧杆菌的红细胞内发育不是必需的。

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来源期刊

Molecular and biochemical parasitology 医学-寄生虫学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

63 days

期刊介绍： The journal provides a medium for rapid publication of investigations of the molecular biology and biochemistry of parasitic protozoa and helminths and their interactions with both the definitive and intermediate host. The main subject areas covered are: • the structure, biosynthesis, degradation, properties and function of DNA, RNA, proteins, lipids, carbohydrates and small molecular-weight substances • intermediary metabolism and bioenergetics • drug target characterization and the mode of action of antiparasitic drugs • molecular and biochemical aspects of membrane structure and function • host-parasite relationships that focus on the parasite, particularly as related to specific parasite molecules. • analysis of genes and genome structure, function and expression • analysis of variation in parasite populations relevant to genetic exchange, pathogenesis, drug and vaccine target characterization, and drug resistance. • parasite protein trafficking, organelle biogenesis, and cellular structure especially with reference to the roles of specific molecules • parasite programmed cell death, development, and cell division at the molecular level.