In vitro fertilization outcomes in a mouse model of gender-affirming hormone therapy in transmasculine youth

Cynthia Dela Cruz Ph.D. , Abigail Wandoff B.S. , Margaret Brunette M.S. , Vasantha Padmanabhan Ph.D. , Ariella Shikanov Ph.D. , Molly B. Moravek M.D., M.P.H.
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Abstract

Objective

To investigate in vitro fertilization (IVF) outcomes in an adolescent transmasculine mouse model mimicking gender-affirming hormone therapy in prepubertal youth, both on testosterone (T) and after T washout.

Design

Experimental laboratory study using a validated mouse model.

Setting

University-based basic science research laboratory.

Animal(s)

A total of 80 prepubertal 26-day-old C57BL/6N female mice were used in this study.

Intervention(s)

Animals (n = 10/group) were implanted subcutaneously with gonadotropin-releasing hormone agonist at 3.6 mg or received sham surgery. After 21 days, they were implanted with silastic tubing containing either T 10 mg or placebo for 6 weeks. After 6 weeks, a group of animals were superovulated for immediate IVF, and another group had the implant removed and went through superovulation for IVF after 2 weeks (washout IVF). The total number of oocytes yielded, oocyte maturity rate, fertilization rate, and numbers of 2-cell embryos, 4–8-cell embryos, morula, blastocysts, and hatching blastocysts were recorded.

Result(s)

Testosterone treatment negatively impacted IVF outcomes in animals stimulated when receiving T, but not after T washout. Pretreatment with gonadotropin-releasing hormone agonist did not affect IVF outcomes.

Conclusion(s)

Although current T had a negative impact on IVF outcomes compared with controls, animals were still able to produce viable oocytes for fertilization and develop into blastocysts. Future efforts to study the impact of long-term T exposure on oocyte quality, especially aneuploidy rates, pregnancy outcomes, and live birth rates, are necessary.

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变性青年性别确认激素治疗小鼠模型的体外受精结果。
目的:研究青春期变性小鼠模型的体外受精(IVF)结果,该模型模拟青春期前青年的性别肯定激素治疗,包括睾酮(T)和T清除后。设计:使用经验证的小鼠模型进行实验性实验室研究。设置:大学基础科学研究实验室。动物:本研究共使用80只26日龄的青春期前C57BL/6N雌性小鼠。干预:动物(n=10/组)皮下植入3.6 mg促性腺激素释放激素激动剂或接受假手术。21天后,他们被植入含有T10 mg T或安慰剂的硅胶管6周。6周后,一组动物进行超排,立即进行试管婴儿,另一组动物移除植入物,并在2周后进行超排试管婴儿(冲洗试管婴儿)。记录产生的卵母细胞总数、卵母细胞成熟率、受精率以及2细胞胚胎、4-8细胞胚胎、桑椹胚、胚泡和孵化胚泡的数量。结果:睾酮治疗对接受T刺激的动物的IVF结果产生了负面影响,但在T冲洗后没有。促性腺激素释放激素激动剂预处理不影响IVF结果。结论:尽管与对照组相比,目前的T对体外受精结果有负面影响,但动物仍然能够产生可存活的卵母细胞进行受精并发育成胚泡。未来有必要研究长期T暴露对卵母细胞质量的影响,特别是非整倍体率、妊娠结局和活产率。
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来源期刊
F&S science
F&S science Endocrinology, Diabetes and Metabolism, Obstetrics, Gynecology and Women's Health, Urology
CiteScore
2.00
自引率
0.00%
发文量
0
审稿时长
51 days
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